Whole Genome Sequencing of the Giant Grouper ( Epinephelus lanceolatus ) and High-Throughput Screening of Putative Antimicrobial Peptide Genes

• Published in: Marine drugs Vol. 17; no. 9; p. 503
• Main Authors: Wang, Dengdong, Chen, Xiyang, Zhang, Xinhui, Li, Jia, Yi, Yunhai, Bian, Chao, Shi, Qiong, Lin, Haoran, Li, Shuisheng, Zhang, Yong, You, Xinxin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.08.2019; MDPI
• Subjects: Adaptive immunity; Animals; Annotations; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial Cationic Peptides - genetics; Antimicrobial Cationic Peptides - immunology; antimicrobial peptide; Antimicrobial peptides; Aquaculture; Bass - genetics; Bass - immunology; Bass - microbiology; Breeding; Breeding - methods; Chromosomes; DNA Shuffling; Economic importance; Economics; Epinephelus; Epinephelus lanceolatus; Fish; Fish Diseases - immunology; Fish Diseases - microbiology; Fish Diseases - prevention & control; Fish Proteins - genetics; Fish Proteins - immunology; Fisheries; Gene Expression Profiling; Gene sequencing; Genes; genome sequencing; Genomes; giant grouper; Glycolysis; growth; High-throughput screening; High-Throughput Screening Assays; Immune response; Marine aquaculture; Marine fishes; Muscles; Pelodiscus sinensis; Peptides; Sequencing; Signal transduction; Thrombin; Transcriptomes; Viral diseases; Viroses; Whole Genome Sequencing; United States > US; China; giant grouper; genome sequencing; Epinephelus lanceolatus; growth; antimicrobial peptide
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md17090503

Giant groupers, the largest grouper type in the world, are of economic importance in marine aquaculture for their rapid growth. At the same time, bacterial and viral diseases have become the main threats to the grouper industry. Here, we report a high-quality genome of a giant grouper sequenced by an Illumina HiSeq X-Ten and PacBio Bioscience Sequel platform. A total of 254 putative antimicrobial peptide (AMP) genes were identified, which can be divided into 34 classes according to the annotation of the Antimicrobial Peptides Database (APD3). Their locations in pseudochromosomes were also determined. Thrombin-, lectin-, and scolopendin-derived putative AMPs were the three largest parts. In addition, expressions of putative AMPs were measured by our transcriptome data. Two putative AMP genes ( and ) were involved in glycolysis, which had extremely high expression levels in giant grouper muscle. As it has been reported that AMPs inhibit the growth of a broad spectrum of microbes and participate in regulating innate and adaptive immune responses, genome sequencing of this study provides a comprehensive cataloging of putative AMPs of groupers, supporting antimicrobial research and aquaculture therapy. These genomic resources will be beneficial to further molecular breeding of this economically important fish.