Overexpressed circPVT1, a potential new circular RNA biomarker, contributes to doxorubicin and cisplatin resistance of osteosarcoma cells by regulating ABCB1

Circular RNAs (circRNAs) represent a widespread class of non-coding RNAs generated from back-splicing, with a circular loop structure. Many circRNAs have been reported to play essential roles in cancer development and have the potential to serve as a novel class of biomarkers for clinical diagnosis....

Full description

Saved in:
Bibliographic Details
Published inInternational journal of biological sciences Vol. 14; no. 3; pp. 321 - 330
Main Authors Kun Peng, Zhu, Long, Xiao, Chun Lin, Zhang
Format Journal Article
LanguageEnglish
Published Australia Ivyspring International Publisher Pty Ltd 01.01.2018
Ivyspring International Publisher
Subjects
Alkaline phosphatase
Biocompatibility
Biomarkers
Bone cancer
Cancer
Circular RNA
Circularity
Cisplatin
Diagnosis
Diagnostic systems
Doxorubicin
Drug resistance
Gastric cancer
Gene expression
Osteosarcoma
Osteosarcoma cells
Research Paper
Ribonucleic acid
RNA
Serum
siRNA
Splicing
circPVT1
chemoresistance
biomarker
circular RNA
osteosarcoma
Online AccessGet full text

Cover

More Information
Summary:Circular RNAs (circRNAs) represent a widespread class of non-coding RNAs generated from back-splicing, with a circular loop structure. Many circRNAs have been reported to play essential roles in cancer development and have the potential to serve as a novel class of biomarkers for clinical diagnosis. However, the role of circRNA in osteosarcoma (OS) remains largely unknown. In the current study, we examined the expression level of circular RNA PVT1 (circPVT1), previously screened and identified the oncogenic role in gastric cancer, in OS and found that circPVT1 was significantly up-regulated in the OS tissues, serums and chemoresistant cell lines, correlated with poor prognosis of OS patients. Besides, ROC curve demonstrated that circPVT1 may be a better diagnostic biomarker than alkaline phosphatase (ALP) in OS with more sensitivity and specificity. In addition, functional assays revealed that circPVT1 knockdown by siRNA could weaken the resistance to doxorubicin and cisplatin of OS cells through decreasing the expression of classical drug resistance-related gene ABCB1. These findings may provide a new insight into the role of circPVT1 as a biomarker for the diagnosis and treatment target of OS.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
Contributed equally to this study and share first authorship.
Conflicts of Interests: We declare that we have no conflicts of interest.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.24360