Characterization of MK₈(H₂) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements

• Published in: Marine drugs Vol. 16; no. 10; p. 391
• Main Authors: Chen, Yashu, Mu, Qin, Hu, Kai, Chen, Mo, Yang, Jifang, Chen, Jigang, Xie, Bijun, Sun, Zhida
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.10.2018; MDPI
• Subjects: antioxidant and antiglycation; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Aquatic Organisms - chemistry; Arctic Regions; Capacity; Cardiovascular disease; Carotenoids; Chromatography, High Pressure Liquid - methods; Countercurrent Distribution - methods; Dietary Supplements; Fermentation; Food; Glycation End Products, Advanced - metabolism; Heat resistance; High performance liquid chromatography; HPLC; Hydrogenation; isoprenoid quinone; Laboratories; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medicine; Menaquinones; Metabolism; Metabolites; Microorganisms; MK8(H2); Molecular docking; Molecular Docking Simulation; NMR; Nuclear magnetic resonance; Phosphorylation; Polar environments; Proteins; Purification; Pyruvaldehyde; Pyruvaldehyde - chemistry; Rhodococcus; Rhodococcus - chemistry; Rhodococcus sp. B7740 from Arctic ocean; Serum; Serum albumin; Tandem Mass Spectrometry - methods; Ubiquinone; Ubiquinone - analogs & derivatives; Ubiquinone - pharmacology; UPLC-HRMS; Vitamin K 2 - chemistry; Vitamin K 2 - isolation & purification; Vitamin K 2 - pharmacology; Vitamins; Water purification; Arctic Regions; China; MK8(H2); NMR; UPLC-HRMS; antioxidant and antiglycation; Rhodococcus sp. B7740 from Arctic ocean; isoprenoid quinone
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md16100391

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK₂), which is mainly produced through the fermentation of microorganisms. MK₈(H₂) was identified to be the main menaquinone from sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK₈(H₂) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK₈(H₂) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK₈(H₂) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK₄. High-performance liquid chromatography⁻mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK₈(H₂) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK₈(H₂) a promising natural active ingredient with future food and medicine applications.