Interactions among Escovopsis , Antagonistic Microfungi Associated with the Fungus-Growing Ant Symbiosis

• Published in: Journal of fungi (Basel) Vol. 7; no. 12; p. 1007
• Main Authors: Christopher, Yuliana, Aguilar, Celestino, Gálvez, Dumas, Wcislo, William T, Gerardo, Nicole M, Fernández-Marín, Hermógenes
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.11.2021; MDPI
• Subjects: antagonism; Attini; Bioassays; coinfection; Colonies; Escovopsis; Fungi; fungus-growing ants; intermingling; Parasites; Phylogenetics; Phylogeny; Symbiosis; vegetative incompatibility; Virulence; Panama; Germany; Attini; vegetative incompatibility; antagonism; fungus-growing ants; coinfection; Escovopsis; intermingling
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof7121007

Fungi in the genus (Ascomycota: Hypocreales) are prevalent associates of the complex symbiosis between fungus-growing ants (Tribe Attini), the ants' cultivated basidiomycete fungi and a consortium of both beneficial and harmful microbes found within the ants' garden communities. Some spp. have been shown to attack the ants' cultivated fungi, and co-infections by multiple spp. are common in gardens in nature. Yet, little is known about how strains impact each other. Since microbe-microbe interactions play a central role in microbial ecology and evolution, we conducted experiments to assay the types of interactions that govern - relationships. We isolated strains from the gardens of 10 attine ant genera representing basal (lower) and derived groups in the attine ant phylogeny. We conducted in vitro experiments to determine the outcome of both intraclonal and interclonal confrontations. When paired with self (intraclonal interactions), isolated from lower attine colonies exhibited antagonistic (inhibitory) responses, while strains isolated from derived attine colonies exhibited neutral or mutualistic interactions, leading to a clear phylogenetic pattern of interaction outcome. Interclonal interactions were more varied, exhibiting less phylogenetic signal. These results can serve as the basis for future studies on the costs and benefits of coinfection, and on the genetic and chemical mechanisms that regulate the compatibility and incompatibility observed here.