ESIPT-based fluorescence probe for the rapid detection of peroxynitrite 'AND' biological thiols

• Published in: Chemical communications (Cambridge, England) Vol. 54; no. 8; pp. 11336 - 11339
• Main Authors: Wu, Luling, Han, Hai-Hao, Liu, Liyuan, Gardiner, Jordan E, Sedgwick, Adam C, Huang, Chusen, Bull, Steven D, He, Xiao-Peng, James, Tony D
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 04.10.2018; Royal Society of Chemistry
• Subjects: Bile; chemical reactions; Chemistry; Chemistry, Multidisciplinary; fluorescence; fluorescent dyes; Fluorescent indicators; image analysis; Medical imaging; permeability; Physical Sciences; rapid methods; Science & Technology; Scientific apparatus & instruments; Thiols; REACTIVE OXYGEN; REDOX REGULATION; GLUTATHIONE; DISEASE; LUMINESCENT; NITRIC-OXIDE; RECENT PROGRESS; STATE; HEALTH; NITRATION
• ISSN: 1359-7345; 1364-548X; 1364-548X
• DOI: 10.1039/c8cc06917d

An ESIPT-based 'AND' logic fluorescence probe ( GSH-ABAH ) was developed for the simultaneous detection of ONOO − and biological thiols. GSH-ABAH was shown to have good cell permeability and with the addition of just SIN-1 (ONOO − donor) or GSH, no fluorescence response was observed in live cells. However, in the presence of both analytes GSH-ABAH could be used to image exogenous ONOO − 'AND' GSH added to RAW264.7 cells. An ESIPT-based 'AND' logic fluorescence probe ( GSH-ABAH ) was developed for the simultaneous detection of ONOO − 'AND' biological thiols.