t(5;14) HOX11L2-positive T-cell acute lymphoblastic leukemia. A collaborative study of the Groupe Français de Cytogénétique Hématologique (GFCH)

• Published in: Leukemia Vol. 17; no. 9; pp. 1851 - 1857
• Main Authors: BERGER, R, DASTUGUE, N, MUGNERET, F, ANDRIEUX, J, TALMANT, P, HELIAS, C, MAUVIEUX, L, LAFAGE-POCHITALOFF, M, MOZZICONACCI, M-J, CORNILLET-LEFEBVRE, P, RADFORD, I, ASNAFI, V, BUSSON, M, BILHOU-NABERA, C, NGUYEN KHAC, F, LEONARD, C, SPELEMAN, F, POPPE, B, BASTARD, C, TAVIAUX, S, QUILICHINI, B, HERENS, C, GREGOIRE, M-J, VAN DEN AKKER, J, CAVE, H, BERNARD, O. A, PEROT, C, BALLERINI, P, HAGEMEIJER, A, MICHAUX, L, CHARRIN, C, PAGES, M. P
• Format: Journal Article Web Resource
• Language: English
• Published: London Nature Publishing 01.09.2003; Nature Publishing Group
• Subjects: 14; Abnormalities; Acute lymphoblastic leukemia; Adolescent; Adult; Adults; Biological and medical sciences; CD3 antigen; Child; Child, Preschool; Children; Chromosome Aberrations; Chromosome translocations; Chromosomes; Chromosomes, Human, Pair 14 - genetics; Chromosomes, Human, Pair 5 - genetics; Clone Cells; Collaboration; Cytogenetics; Female; Fluorescence; Fluorescence in situ hybridization; Hematologic and hematopoietic diseases; Hematology; Homeodomain Proteins - genetics; HOX11L2; Human health sciences; Humans; Hématologie; Immunophenotyping; In Situ Hybridization, Fluorescence; Karyotypes; Karyotyping; Leukemia; Leukemia-Lymphoma, Adult T-Cell - genetics; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphatic leukemia; Lymphocytes T; Male; Medical sciences; Oncogene Proteins - genetics; Oncologie; Oncology; Ploidies; Proto-Oncogene Proteins; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; Sciences de la santé humaine; Survival Rate; T-cell ALL; Translocation; Translocation, Genetic; Belgium; France; Paris France; Chromosomal aberration; Human; t5; 14; Acute; HOX11L2; Malignant hemopathy; T-cell ALL; Abnormal chromosome B5; acute lymphoblastic leukemia; Lymphoproliferative syndrome; T-Lymphocyte; Gene rearrangement; Precursor T lymphoblastic leukemia; Abnormal chromosome D14; Abnormal chromosome; Genetics; Adult; Acute lymphocytic leukemia; Child; Hybrid gene; Chromosome translocation
• ISSN: 0887-6924; 1476-5551; 1476-5551
• DOI: 10.1038/sj.leu.2403061

To accurately estimate the incidence of HOX11L2 expression, and determine the associated cytogenetic features, in T-cell acute lymphoblastic leukemia (T-ALL), the Groupe Français de Cytogénétique Hématologique (GFCH) carried out a retrospective study of both childhood and adult patients. In total, 364 patients were included (211 children </=15 years and 153 adults), and 67 (18.5%) [47 children (22.4%) and 20 adults (13.1%)] were shown to either harbor the t(5;14)q35;q32) translocation or express the HOX11L2 gene or both. Most of the common hematological parameters did not show significant differences within positive and negative populations, whereas the incidence of CD1a+/CD10+ and cytoplasmic CD3+ patients was significantly higher in positive than in negative children. Out of the 63 positive patients investigated by conventional cytogenetics, 32 exhibited normal karyotype, whereas the others 31 showed clonal chromosome abnormalities, which did not include classical T-ALL specific translocations. Involvement of the RANBP17/HOX11L2 locus was ascertained by fluorescence in situ hybridization in six variant or alternative (three-way translocation or cytogenetic partner other than 14q32) translocations out of the 223 patients. Our results also show that HOX11L2 expression essentially occurs as a result of a 5q35 rearrangement, but is not associated with another identified T-ALL specific recurrent genetic abnormality, such as SIL-TAL fusion or HOX11 expression.