Epithelial–mesenchymal transition, and collective and individual cell migration regulate epithelial changes in the amikacin-damaged organ of Corti

Characterizing the microenvironment of a damaged organ of Corti and identifying the basic mechanisms involved in subsequent epithelial reorganization are critical for improving the outcome of clinical therapies. In this context, we studied the expression of a variety of cell markers related to cell...

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Bibliographic Details
Published inHistochemistry and cell biology Vol. 148; no. 2; pp. 129 - 142
Main Authors Ladrech, Sabine, Eybalin, Michel, Puel, Jean-Luc, Lenoir, Marc
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2017
Springer Nature B.V
Springer Verlag
Subjects
Amikacin
Amikacin - pharmacology
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell adhesion
Cell adhesion & migration
Cell Biology
Cell migration
Cell Movement - drug effects
Cell size
Developmental Biology
Ear diseases
Ears & hearing
Epithelial Cells - drug effects
Epithelial Cells - pathology
Epithelial-Mesenchymal Transition - drug effects
Epithelium
Fibroblasts
Homeostasis
Life Sciences
Mesenchyme
Neurobiology
Neurons and Cognition
Organ of Corti
Organ of Corti - drug effects
Organ of Corti - pathology
Original Paper
Rats
Rats, Wistar
Cochlea
Fibroblasts
Flat epithelium
Root cells
Adhesion proteins
Ototoxicity
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Summary:Characterizing the microenvironment of a damaged organ of Corti and identifying the basic mechanisms involved in subsequent epithelial reorganization are critical for improving the outcome of clinical therapies. In this context, we studied the expression of a variety of cell markers related to cell shape, cell adhesion and cell plasticity in the rat organ of Corti poisoned with amikacin. Our results indicate that, after severe outer hair cell losses, the cytoarchitectural reorganization of the organ of Corti implicates epithelial-mesenchymal transition mechanisms and involves both collective and individual cell migratory processes. The results also suggest that both root cells and infiltrated fibroblasts participate in the homeostasis of the damaged epithelium, and that the flat epithelium that may emerge offers biological opportunities for late regenerative therapies.
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-017-1548-6