Protective Effects of 3,4-Oxo-isopropylidene-Shikimic Acid on Experimental Colitis Induced by Trinitrobenzenesulfonic Acid in Rats

• Published in: Digestive diseases and sciences Vol. 57; no. 8; pp. 2045 - 2054
• Main Authors: Xing, Jian-Feng, Sun, Jian-Ning, Sun, Jin-Yao, You, Cui-Yu, Dong, Kai, Lv, Jun, Dong, Ya-Lin
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.08.2012; Springer; Springer Nature B.V
• Subjects: Animals; Biochemistry; Colitis; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Colon - immunology; Colon - metabolism; Colon - pathology; Disease Models, Animal; Drug Evaluation, Preclinical; Gastroenterology; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Hepatology; Illicium; Inflammation; Insomnia; Male; Malondialdehyde - metabolism; Medicine; Medicine & Public Health; Medicine, Chinese; Neutrophil Infiltration; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - metabolism; Oncology; Original Article; Rats; Rats, Sprague-Dawley; Resveratrol; Shikimic Acid - analogs & derivatives; Shikimic Acid - pharmacology; Shikimic Acid - therapeutic use; Skin; Superoxide; Superoxide Dismutase - metabolism; Transplant Surgery; Trinitrobenzenesulfonic Acid; Experimental colitis; 3,4-Oxo-isopropylidene-shikimic acid; 2,4,6-Trinitrobenzenesulfonic acid; Antioxidation
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-012-2155-y

Background 3,4-Oxo-isopropylidene-shikimic acid (ISA) is a derivative of shikimic acid (SA). SA is extracted from Illicium verum Hook.fil., which has been used in traditional Chinese medicine and used for treating vomiting, stomach aches, insomnia, skin inflammation, and rheumatic pain. Aims To investigate the effects and the protective mechanism of 3,4-oxo-isopropylidene-shikimic acid on experimental colitis model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats. Methods Colitis in rats was induced by colonic administration with TNBS. ISA (50, 100, and 200 mg/kg) was administered for 12 days to experimental colitis rats. The inflammatory degree was assessed by macroscopic damage score, colon weight/length ratios (mg/cm), and myeloperoxidase (MPO) activity. Malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) levels, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS) activities were measured with biochemical methods. Results ISA significantly ameliorated macroscopic damage, reduced colon weight/length ratios and the activity of MPO, depressed MDA and NO levels and iNOS activity, and enhanced GSH level, and GSH-Px and SOD activities in the colon tissues of experimental colitis in a dose-dependent manner. Moreover, the effect of ISA (200 mg/kg) was as effective as sulfasalazine (500 mg/kg). Conclusions The findings of this study demonstrate the protective effect of ISA on experimental colitis, probably due to an antioxidant action.