Association of endothelial nitric oxide synthase (eNOS) gene G894T polymorphism with hypertension risk and complications

• Published in: Molecular and cellular biochemistry Vol. 421; no. 1-2; pp. 103 - 110
• Main Authors: ALrefai, Abeer A., Habib, Mona Salah El-din, Yaseen, Rehab I., Gabr, Mahmoud K., Habeeb, Rabab M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2016; Springer; Springer Nature B.V
• Subjects: Adult; Aged; Alleles; Amino Acid Substitution; Biochemistry; Biomedical and Life Sciences; Biosynthesis; Cardiology; Cardiovascular disease; Endothelium; Female; Gene Frequency; Genetic aspects; Genetic polymorphisms; Humans; Hypercholesterolemia; Hypertension; Hypertension - enzymology; Hypertension - epidemiology; Hypertension - genetics; Life Sciences; Male; Medical Biochemistry; Middle Aged; Mutation, Missense; Nitric oxide; Nitric Oxide Synthase Type III - genetics; Oncology; Polymorphism; Polymorphism, Genetic; Risk Factors; Type 2 diabetes; Hypertension; CVD; G894T; eNOS (G894T)
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-016-2790-2

This study evaluated the association of NOS3 polymorphisms with hypertension risk and complications. eNOS (G894T) SNP was performed by RT-PCR on 70 hypertensive patients (25 were hypertensive, 25 were hypertensive with CAD, and 20 were diabetic with hypertension) and 30 age- and gender-matched individuals. Lipid and glucose profile were assessed by standard colorimetric assay. Our results revealed that combination of (GT + TT) genotype and T allele significantly increases the risk of hypertension (OR = 3.86 and 4.33), respectively. Subgroup analysis showed significant association between CAD with eNOS (G894T) mutant genotype ( P  = 0.002) and allele frequency ( P  < 0.001). Moreover, the mutant homozygous and heterozygous eNOS genotype together were significantly associated with higher TC, LDLc, ( P  < 0.001), and TG ( P  = 0.001). Thus, hypercholesterolemia ( P  < 0.001 and OR = 12.48) increases the risk of hypertension among T carrier. These results indicated that the T carriers significantly increase hypertension risk and complication (CAD), mainly with hypercholesterolemia and in elderly.