New Sorbicillinoids with Tea Pathogenic Fungus Inhibitory Effect from Marine-Derived Fungus Hypocrea jecorina H8

• Published in: Marine drugs Vol. 20; no. 3; p. 213
• Main Authors: Liu, Shun-Zhi, Xu, Guang-Xin, He, Feng-Ming, Zhang, Wei-Bo, Wu, Zhen, Li, Ming-Yu, Tang, Xi-Xiang, Qiu, Ying-Kun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.03.2022; MDPI
• Subjects: Analogs; Animal embryos; Animals; Ascomycota - drug effects; Ascomycota - growth & development; Biological Control Agents - chemistry; Biological Control Agents - isolation & purification; Biological Control Agents - pharmacology; Biological Control Agents - toxicity; Camellia sinensis - microbiology; Carbon; Danio rerio; Embryo, Nonmammalian; Embryos; Freshwater fishes; Fungi; Hypocrea jecorina H8; Hypocreales - chemistry; Larvae; Mangroves; Metabolites; Molecular Structure; NMR; Nuclear magnetic resonance; Ova; Pathogens; Planar structures; Polyketides - chemistry; Polyketides - isolation & purification; Polyketides - pharmacology; Polyketides - toxicity; Tea; tea pathogenic fungus inhibitory effect; Toxicity; toxicity assessment; trichodermolide C, D; Zebrafish; Hypocrea jecorina H8; toxicity assessment; tea pathogenic fungus inhibitory effect; trichodermolide C, D
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20030213

Four new dimeric sorbicillinoids ( - and ) and a new monomeric sorbicillinoid ( ) as well as six known analogs ( - ) were purified from the fungal strain H8, which was obtained from mangrove sediment, and showed potent inhibitory activity against the tea pathogenic fungus ( ). The planar structures of - were assigned by analyses of their UV, IR, HR-ESI-MS, and NMR spectroscopic data. All the compounds were evaluated for growth inhibition of tea pathogenic fungus . Compounds , , , , and exhibited more potent inhibitory activities compared with the positive control hexaconazole with an ED of 24.25 ± 1.57 µg/mL. The ED values of compounds , , , , and were 9.13 ± 1.25, 2.04 ± 1.24, 18.22 ± 1.29, 1.83 ± 1.37, and 4.68 ± 1.44 µg/mL, respectively. Additionally, the effects of these compounds on zebrafish embryo development were also evaluated. Except for compounds and , which imparted toxic effects on zebrafish even at 0.625 μM, the other isolated compounds did not exhibit significant toxicity to zebrafish eggs, embryos, or larvae. Taken together, sorbicillinoid derivatives ( , , and ) from H8 displayed low toxicity and high anti-tea pathogenic fungus potential.