Anti-Proliferation Activity of a Decapeptide from Perinereies aibuhitensis toward Human Lung Cancer H1299 Cells

• Published in: Marine drugs Vol. 17; no. 2; p. 122
• Main Authors: Jiang, Shuoqi, Jia, Yinglu, Tang, Yunping, Zheng, Die, Han, Xingbiao, Yu, Fangmiao, Chen, Yan, Huang, Fangfang, Yang, Zuisu, Ding, Guofang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.02.2019; MDPI
• Subjects: Amino Acid Sequence; Animals; Anticancer properties; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis; Apoptosis - drug effects; Apoptosis Regulatory Proteins - drug effects; Apoptosis Regulatory Proteins - metabolism; Cancer; Cancer therapies; Caspase; Caspases - metabolism; Cell cycle; Cell Cycle - drug effects; Cell growth; Cell Line, Tumor; Cell morphology; Cell proliferation; Cell Proliferation - drug effects; Cells; Chemotherapy; Cytology; Cytotoxicity; decapeptide; Dietary supplements; Dose-Response Relationship, Drug; Drug dosages; Flow cytometry; Functional foods & nutraceuticals; Growth factors; Humans; Hydrolysates; Lung cancer; Lung Neoplasms - drug therapy; Lungs; Morphology; Mortality; Neoplasm Metastasis - prevention & control; Non-small cell lung carcinoma; Nucleoside-diphosphate kinase; Oligopeptides - isolation & purification; Oligopeptides - pharmacology; Peptides; Perinereis aibuhitensis; Polychaeta - chemistry; Proliferation; Prostate cancer; Proteins; S phase; Small cell lung carcinoma; Tumors; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - biosynthesis; Western blotting; lung cancer; apoptosis; Perinereis aibuhitensis; cell proliferation; decapeptide
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md17020122

peptide (PAP) is a decapeptide (Ile-Glu-Pro-Gly-Thr-Val-Gly-Met-Met-Phe, IEPGTVGMMF) with anticancer activity that was purified from an enzymatic hydrolysate of . In the present study, the anticancer effect of PAP on H1299 cell proliferation was investigated. Our results showed that PAP promoted apoptosis and inhibited the proliferation of H1299 cells in a time- and dose-dependent manner. When the PAP concentration reached 0.92 mM, more than 95% of treated cells died after 72 h of treatment. Changes in cell morphology were further analyzed using an inverted microscope and AO/EB staining and flow cytometry was adopted for detecting apoptosis and cell cycle phase. The results showed that the early and late apoptosis rates of H1299 cells increased significantly after treatment with PAP and the total apoptosis rate was significantly higher than that of the control group. Moreover, after treatment with PAP, the number of cells in the S phase of cells was significantly reduced and the ability for the cells to proliferate was also reduced. H1299 cells were arrested in the G2/M phase and cell cycle progression was inhibited. Furthermore, the results of western blotting showed that nm23-H1 and vascular endothelial growth factor (VEGF) protein levels decreased in a dose-dependent manner, while the pro-apoptotic protein and anti-apoptotic protein ratios and the level of apoptosis-related caspase protein increased in a dose-dependent manner. In conclusion, our results indicated that PAP, as a natural marine bioactive substance, inhibited proliferation and induced apoptosis of human lung cancer H1299 cells. PAP is likely to be exploited as the functional food or adjuvant that may be used for prevention or treatment of human non-small cell lung cancer in the future.