Detectable expression of IL-35 in CD4+ T cells from peripheral blood of chronic Hepatitis B patients

• Published in: Clinical immunology (Orlando, Fla.) Vol. 139; no. 1; pp. 1 - 5
• Main Authors: Liu, Fen, Tong, Fuyi, He, Yan, Liu, Haiyan
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.04.2011; Elsevier
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Adult; Allergy and Immunology; Biological and medical sciences; Blood Donors; Blotting, Western; Case-Control Studies; CD4+ T cells; CD4-Positive T-Lymphocytes - metabolism; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression Regulation - immunology; Hepatitis B; Hepatitis B virus; Hepatitis B, Chronic - blood; Human viral diseases; Humans; IL-35; Immunoprecipitation; Infectious diseases; Interleukins - genetics; Interleukins - metabolism; Male; Medical sciences; Middle Aged; Minor Histocompatibility Antigens; Regulatory T cells; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Viral diseases; Viral hepatitis; Young Adult; CD4 + T cells; IL-35; Regulatory T cells; Hepatitis B; CD4+ T cells; Human; CD4 T lymphocyte; Cytokine; Hepatic disease; T cells; Blood; Infection; Viral hepatitis B; CD4; Immunology; Viral disease; T-Lymphocyte; Digestive diseases; Regulatory cell
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1016/j.clim.2010.12.012

Abstract Epstein–Barr virus-induced gene 3 (Ebi3) and the p35 subunit of IL-12 have been reported to form a heterodimeric cytokine, named IL-35, in human and mouse. In mice, IL-35 has been shown to be constitutively expressed by CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) and suggested to contribute to their suppressive activity. However, human CD4+ CD25+ Foxp3+ Tregs do not constitutively express detectable amounts of IL-35 in both mRNA and protein levels. Circulating CD4+ CD25+ Treg frequency of chronic Hepatitis B patients significantly correlates with serum viral load. In this study, we investigated whether IL-35 expression could be detected in CD4+ T cells from peripheral blood of chronic Hepatitis B patients. Using both RT-PCR and immunoprecipitation plus Western blot analysis, we demonstrated that IL-35 expression could be detected in the CD4+ T cells from peripheral blood of Chronic Hepatitis B patients.