Extraction of polysaccharides and its antitumor activity on Magnolia kwangsiensis Figlar & Noot

• Published in: Carbohydrate polymers Vol. 142; pp. 98 - 104
• Main Authors: Zheng, Yan-Fei, Zhang, Qiang, Liu, Xiong-Min, Ma, Li, Lai, Fang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 20.05.2016
• Subjects: Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Antitumor; Cell Line, Tumor; Cell Proliferation - drug effects; Extraction; Humans; Lung - drug effects; Lung - pathology; Lung Neoplasms - drug therapy; Lung Neoplasms - pathology; Magnolia - chemistry; Magnolia kwangsiensis Figlar & Noot; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Stomach - drug effects; Stomach - pathology; Stomach Neoplasms - drug therapy; Stomach Neoplasms - pathology; Polysaccharides; Extraction; Magnolia kwangsiensis Figlar & Noot; Antitumor
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2016.01.039

•The crude polysaccharides from the leaves of Magnolia kwangsiensis Figlar & Noot. were extracted by hot water extraction.•Two homogeneous polysaccharides were obtained by DEAE-52 cellulose chromatography and Sephadex G-100 column chromatography.•P-2 and P-3 were characterized by IR, HPGPC and GC–MS.•The antitumor activities of P-2 and P-3 were evaluated.•P-3 with stronger antitumor activities was found. The crude polysaccharides from the leaves of Magnolia kwangsiensis Figlar & Noot. were extracted by hot water extraction, the yield was 5.09%. Two polysaccharide fractions (P-2 and P-3) were isolated by DEAE-52 cellulose chromatography and Sephadex G-100 column chromatography in order, respectively. P-2 and P-3 were characterized by IR, HPGPC and GC–MS. P-2 was comprised of only glucose. Its molecular weight was 11.2×103Da and the formula was C414H690O345. P-3 was comprised of xylose and rhamnose in the ratio of 1:4. Its molecular weight was 7.8×103Da, and the formula was C319H528O220. The antitumor activities of P-2 and P-3 on the growth of human lung cancer (A549) cells and human gastric carcinoma (SGC7901) cells in vitro were evaluated. The results indicated that P-3 exhibited marked antitumor activities with IC50 value of 8.48 and 5.66μg/mL.