Anti-Inflammatory Activity of Fucoidan Extracts In Vitro

• Published in: Marine drugs Vol. 19; no. 12; p. 702
• Main Authors: Ahmad, Tauseef, Eapen, Mathew Suji, Ishaq, Muhammad, Park, Ah Young, Karpiniec, Samuel S, Stringer, Damien N, Sohal, Sukhwinder Singh, Fitton, J Helen, Guven, Nuri, Caruso, Vanni, Eri, Rajaraman
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.12.2021; MDPI
• Subjects: Algae; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Aquatic Organisms; Biocompatibility; Cell growth; Cytokines; Cytotoxicity; Fucoidan; Fucus vesiculosus; Humans; IL-1β; In vivo methods and tests; Incubation; Incubation period; Inflammation; Inflammatory bowel disease; Interleukin 6; Interleukin-1 - metabolism; Interleukin-6 - metabolism; Leukocytes (mononuclear); Leukocytes, Mononuclear - drug effects; Lipopolysaccharides; Low concentrations; Macrocystis pyrifera; Macrophages; Macrophages - drug effects; Metabolism; Molecular weight; Pathology; PBMCs; Peripheral blood mononuclear cells; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polymers; Polysaccharides - chemistry; Polysaccharides - pharmacology; Seaweed; Seaweeds; THP-1; TNF-α, IL-1β, and IL-6; Toxicity; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Undaria pinnatifida; Fucus vesiculosus; THP-1; Laminaria japonica; Macrocystis pyrifera; Undaria pinnatifida; TNF-α, IL-1β, and IL-6; PBMCs; fucoidan; Ascophyllum nodosum
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md19120702

Fucoidans are sulfated, complex, fucose-rich polymers found in brown seaweeds. Fucoidans have been shown to have multiple bioactivities, including anti-inflammatory effects, and are known to inhibit inflammatory processes via a number of pathways such as selectin blockade and enzyme inhibition, and have demonstrated inhibition of inflammatory pathologies . In this current investigation, fucoidan extracts from and were assessed for modulation of pro-inflammatory cytokine production (TNF-α, IL-1β, and IL-6) by human peripheral blood mononuclear cells (PBMCs) and in a human macrophage line (THP-1). Fucoidan extracts exhibited no signs of cytotoxicity in THP-1 cells after incubation of 48 h. Additionally, all fucoidan extracts reduced cytokine production in LPS stimulated PBMCs and human THP-1 cells in a dose-dependent fashion. Notably, the 5-30 kDa subfraction from was a highly effective inhibitor at lower concentrations. Fucoidan extracts from all species had significant anti-inflammatory effects, but the lowest molecular weight subfractions had maximal effects at low concentrations. These observations on various fucoidan extracts offer insight into strategies that improve their efficacy against inflammation-related pathology. Further studies should be conducted to elucidate the mechanism of action of these extracts.