Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis via Suppression of C-C Chemokine Receptor Type 7 Expression

• Published in: Nutrients Vol. 11; no. 2; p. 410
• Main Authors: Kaya, Pelin, Lee, Sang R, Lee, Young Ho, Kwon, Sun Woo, Yang, Hyun, Lee, Hye Won, Hong, Eui-Ju
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.02.2019; MDPI
• Subjects: Activator protein 1; Animals; Antineoplastic Agents - pharmacology; Breast cancer; c-Fos protein; c-Jun protein; CC chemokine receptors; CCL21 protein; CCR7; CCR7 protein; Cell adhesion & migration; Chemokines; Chemotherapy; Chronic lymphocytic leukemia; Curcuma; curcumae radix; Curcumin; Disruption; Ethanol; Extracellular signal-regulated kinase; Female; Forkhead protein; Foxp3 protein; Gelatinase B; Gene expression; Genes, fos - drug effects; Genes, jun - drug effects; Kinases; Leukemia; Lung cancer; Lung Neoplasms - prevention & control; Lung Neoplasms - secondary; Lungs; Lymphatic leukemia; Lymphocytes B; Lymphocytes T; Mammary gland; Mammary Neoplasms, Experimental - drug therapy; Mammary Neoplasms, Experimental - pathology; Matrix Metalloproteinase 9 - drug effects; Medical prognosis; Medicine; Metastases; Metastasis; Mice; Mice, Transgenic; MMP9; Neoplasm Metastasis - prevention & control; Plant Extracts - pharmacology; Plant Roots; PyMT/MMTV; Quality of life; Quantitative analysis; Receptors, CCR7 - biosynthesis; Receptors, CCR7 - drug effects; Survival analysis; Transcription factors; Tumors; United States > US; South Korea; breast cancer; CCR7; PyMT/MMTV; MMP9; curcumae radix
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11020410

Curcumae radix is the dry root of (turmeric) that can be used either as a spice or traditional medicine. The aim of this study was to investigate the survival benefits and the anti-metastatic activity of curcumae radix extract (CRE) in MCF7 cells and in MMTV-PyMT transgenic mice-a mouse model of breast cancer metastasis. In vitro wound scratch assay revealed that CRE treatment inhibited cell motility and cell migration in a dose-dependent manner. To investigate the effect of CRE in breast cancer metastasis, MMTV-PyMT transgenic female virgin mice were used and randomly divided into two groups. For survival curve analysis, CRE was administered in a dose of 50 mg/kg to 8⁻20-week-old mice. Interestingly, CRE treatment significantly increased the median and prolonged survival of MMTV-PyMT mice. Furthermore, CRE treatment decreased tumor burden and inhibited cell proliferation in primary breast tumor, and also suppressed mammary tumor-derived lung metastasis. The size of the lung metastases substantially decreased in the CRE-treated group compared with the ones in the control group. Curcumae radix extract showed anti-metastatic activity through regulating the expression of metastasis markers including C-C Chemokine Receptor Type 7, Matrix Metalloproteinase 9 and the proto-oncogenes c-fos and c-jun. We demonstrated that these metastatic regulators were decreased when CCR7 expression was suppressed in MCF7 cells transfected with CCR7 siRNA. The results of this study show that curcumae radix exerts antitumor and anti-metastatic activities, and we suggest that curcumae radix might be a potential supplement for the treatment and prevention of breast cancer metastasis.