Collagen Extracted from Bigeye Tuna ( Thunnus obesus ) Skin by Isoelectric Precipitation: Physicochemical Properties, Proliferation, and Migration Activities

• Published in: Marine drugs Vol. 17; no. 5; p. 261
• Main Authors: Lin, Xinhui, Chen, Yinyue, Jin, Huoxi, Zhao, Qiaoling, Liu, Chenjuan, Li, Renwei, Yu, Fangmiao, Chen, Yan, Huang, Fangfang, Yang, Zuisu, Ding, Guofang, Tang, Yunping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.05.2019; MDPI
• Subjects: Amino acids; Analytical methods; Biomedical materials; By products; Collagen; Collagen (type I); Dry weight; Electrophoresis; Fibroblasts; Gel electrophoresis; Gels; Heavy metals; Hemodialysis; Inductively coupled plasma mass spectrometry; isoelectric precipitation; Mass spectrometry; Mass spectroscopy; Metals; Physicochemical processes; Physicochemical properties; Polyacrylamide; Precipitation; Proliferation; proliferation and migration; Proteins; Salt; Salting; Skin; Sodium; Sodium dodecyl sulfate; Sodium lauryl sulfate; Spectrum analysis; Thunnus obesus; Tuna; Vibration; Weight; Yields; isoelectric precipitation; Thunnus obesus; collagen; physicochemical properties; proliferation and migration
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md17050261

Collagen was extracted from bigeye tuna ( ) skins by salting-out (PSC-SO) and isoelectric precipitation (PSC-IP) methods. The yield of the PSC-IP product was approximately 17.17% (dry weight), which was greater than the yield obtained from PSC-SO (14.14% dry weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that collagen from bigeye tuna skin belongs to collagen type I. Inductively coupled plasma mass spectrometry results indicate that the heavy metal abundance in PSC-IP was lower than the maximum acceptable amounts according to Chinese regulatory standards. In addition, results from a methylthiazolyldiphenyl-tetrazolium bromide assay and an in vitro scratch assay demonstrated that PSC-IP could promote the proliferation and migration of NIH-3T3 fibroblasts. Overall, results suggest PSC-IP could be used to rapidly extract collagen from marine by-products instead of traditional salting-out methods. Collagen from bigeye tuna skin may also have strong potential for cosmetic and biomedical applications.