Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization

Several proteins have been crosslinked to DNA by low dose uv irradiation. The principle of the method is based on an efficient and fast radiation induced reaction of amino acid residues with DNA at low pH. The method seems to be of general applicability for crosslinking proteins to DNA in a very sim...

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Bibliographic Details
Published inNucleic acids research Vol. 17; no. 4; pp. 1563 - 1572
Main Authors CZICHOS, J, KÖHLER, M, RECKMANN, B, RENZ, M
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.02.1989
Subjects
Biological and medical sciences
Blotting, Southern
Cross-Linking Reagents
Diverse techniques
DNA, Bacterial - radiation effects
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Nucleic Acid Hybridization
Plasmids - radiation effects
proteins
Proteins - radiation effects
Staphylococcal Protein A - radiation effects
U.V. radiation
Ultraviolet Rays
Molecular hybridization
Binding protein
Ultraviolet irradiation
DNA
Molecular interaction
Crosslinking
Genetic engineering
Molecular probe
Method
Conjugated compound
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Summary:Several proteins have been crosslinked to DNA by low dose uv irradiation. The principle of the method is based on an efficient and fast radiation induced reaction of amino acid residues with DNA at low pH. The method seems to be of general applicability for crosslinking proteins to DNA in a very simple one step procedure. Some of such DNA-protein conjugates have been used as probes for hybridization experiments. DNA-protein A probes were found to be most useful.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/17.4.1563