Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp

• Published in: The plant pathology journal Vol. 39; no. 6; pp. 600 - 613
• Main Authors: Shin, Jong-Hwan, Lee, Ha-Kyoung, Lee, Seong-Chan, Han, You-Kyoung
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society of Plant Pathology 01.12.2023; Hanrimwon Publishing Company
• Subjects: bacillus thuringiensis; biological control; fusarium basal rot; fusarium oxysporum; onion disease; onion disease; biological control; Fusarium oxysporum; Fusarium basal rot; Bacillus thuringiensis
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.OA.08.2023.0118

Fusarium oxysporum is the main pathogen causing Fusarium basal rot in onion (Allium cepa L.), which incurs significant yield losses before and after harvest. Among management strategies, biological control is an environmentally safe and sustainable alternative to chemical control. In this study, we isolated and screened bacteria for antifungal activity against the basal rot pathogen F. oxysporum. Isolates 23-045, 23-046, 23-052, 23-055, and 23-056 significantly inhibited F. oxysporum mycelial growth and conidial germination. Isolates 23-045, 23-046, 23-052, and 23-056 suppressed the development of Fusarium basal rot in both onion seedlings and bulbs in pot and spray inoculation assays. Isolate 23-055 was effective in onion seedlings but exhibited weak inhibitory effect on onion bulbs. Based on analyses of the 16S rRNA and rpoB gene sequences together with morphological analysis, isolates 23-045, 23-046, 23-052, and 23-055 were identified as Bacillus thuringiensis, and isolate 23-056 as Bacillus toyonensis. All five bacterial isolates exhibited cellulolytic, proteolytic, and phosphate-solubilizing activity, which may contribute to their antagonistic activity against onion basal rot disease. Taken together B. thuringiensis 23-045, 23-046, 23-052, and 23-055 and B. toyonensis 23-056 have potential for the biological control of Fusarium basal rot in onion.