Proteomics Analysis Reveals Post-Translational Mechanisms for Cold-Induced Metabolic Changes in Arabidopsis

• Published in: Molecular plant Vol. 4; no. 2; pp. 361 - 374
• Main Authors: Li, Tian, Xu, Shou-Ling, Oses-Prieto, Juan A., Putil, Sunita, Xu, Peng, Wang, Rui-Ju, Li, Kathy H., Maltby, David A., An, Liz-He, Burlingame, Alma L., Deng, Zhi-Ping, Wang, Zhi-Yong
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.03.2011; Cell Press; Oxford University Press
• Subjects: 2-D DIGE; Acclimatization; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Centrifugation; Cold; Cold response; Cold Temperature; Enzymatic activity; Freezing; Freezing point; freezing tolerance; Gene expression; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; heat shock protein; Inactivation; Low temperature; Mass spectrometry; Plant protection; Proteins; Proteomics; Proteomics - methods; starch metabolism; Two-Dimensional Difference Gel Electrophoresis; 代谢变化; 冷诱导; 分子机制; 学分; 拟南芥; 植物生存; 蛋白质组; 转化; heat shock protein; Cold response; starch metabolism; 2-D DIGE; Arabidopsis; freezing tolerance
• ISSN: 1674-2052; 1752-9867; 1752-9867
• DOI: 10.1093/mp/ssq078

Cold-induced changes of gene expression and metabolism are critical for plants to survive freezing. Largely by changing gene expression, exposure to a period of non-freezing low temperatures increases plant tolerance to freezing--a phenomenon known as cold acclimation. Cold also induces rapid metabolic changes, which provide instant protection before temperature drops below freezing point. The molecular mechanisms for such rapid metabolic responses to cold remain largely unknown. Here, we use two-dimensional difference gel electrophoresis （2-D DIGE） analysis of sub-cellular fractions ofArabidopsis thaliana proteome coupled with spot identification by tandem mass spectrometry to identify early cold-responsive proteins in Arabidopsis. These proteins include four enzymes involved in starch degradation, three HSP100 proteins, several proteins in the tricarboxylic acid cycle, and sucrose metabolism. Upon cold treatment, the Disproportio- nating Enzyme 2 （DPE2）, a cytosolic transglucosidase metabolizing maltose to glucose, increased rapidly in the centrifugation pellet fraction and decreased in the soluble fraction. Consistent with cold-induced inactivation of DPE2 enzymatic activity, the dpe2 mutant showed increased freezing tolerance without affecting the C-repeat binding transcription factor （CBF） transcriptional pathway. These results support a model that cold-induced inactivation of DPE2 leads to rapid accumulation of maltose, which is a cold-induced compatible solute that protects cells from freezing damage. This study provides evidence for a key role of rapid post-translational regulation of carbohydrate metabolic enzymes in plant protection against sudden temperature drop.