Physicochemical and Antioxidant Properties of Acid- and Pepsin-Soluble Collagens from the Scales of Miiuy Croaker ( Miichthys Miiuy )

• Published in: Marine drugs Vol. 16; no. 10; p. 394
• Main Authors: Li, Long-Yan, Zhao, Yu-Qin, He, Yu, Chi, Chang-Feng, Wang, Bin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.10.2018; MDPI
• Subjects: acid-soluble collagen (ASC); Acids - chemistry; Amino acid composition; Amino acids; Amino Acids - chemistry; Analytical methods; Animal Scales - chemistry; Animals; Anions; antioxidant activity; Antioxidant properties; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Aquaculture; Collagen; Collagen (type I); Collagen - chemistry; Collagen - isolation & purification; Collagen - pharmacology; Collagen - ultrastructure; Cosmeceuticals; Denaturation; Disease; Dry weight; Environmental impact; Fish Proteins - chemistry; Fish Proteins - isolation & purification; Fish Proteins - pharmacology; Fish Proteins - ultrastructure; Food; Fourier transforms; Free Radical Scavengers; Free Radicals; Gel electrophoresis; Glycine; Glycine (amino acid); Hydrogen-Ion Concentration; Hydroxyl radicals; Infrared spectra; Infrared spectroscopy; Microscopy, Electron, Scanning; miiuy croaker (Miichthys miiuy); Moisture absorption; Molecular weight; Pepsin; Pepsin A - chemistry; pepsin-soluble collagen (PSC); Perciformes; pH effects; Polypeptides; Protein denaturation; Proteins; radical scavenging activity; Residues; scale; Scavenging; Skin; Sodium chloride; Sodium lauryl sulfate; Solubility; Spectroscopy, Fourier Transform Infrared; Sulfonic acid; Superoxide; Ultraviolet radiation; Weight; Zeta potential; China; acid-soluble collagen (ASC); scale; radical scavenging activity; pepsin-soluble collagen (PSC); antioxidant activity; miiuy croaker (Miichthys miiuy)
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md16100394

In this report, acid-soluble collagen (ASC-MC) and pepsin-soluble collagen (PSC-MC) were extracted from the scales of miiuy croaker ( ) with yields of 0.64 ± 0.07% and 3.87 ± 0.15% of dry weight basis, respectively. ASC-MC and PSC-MC had glycine as the major amino acid with the contents of 341.8 ± 4.2 and 344.5 ± 3.2 residues/1000 residues, respectively. ASC-MC and PSC-MC had lower denaturation temperatures (32.2 °C and 29.0 °C for ASC-MC and PSC-MC, respectively) compared to mammalian collagen due to their low imino acid content (197.6 and 195.2 residues/1000 residues for ASC-MC and PSC-MC, respectively). ASC-MC and PSC-MC were mainly composed of type I collagen on the literatures and results of amino acid composition, SDS-PAGE pattern, ultraviolet (UV) and Fourier-transform infrared spectroscopy (FTIR) spectra. The maximum solubility of ASC-MC and PSC-MC was appeared at pH 1⁻3 and a sharp decrease in solubility was observed when the NaCl concentration was above 2%. Zeta potential studies indicated that ASC-MC and PSC-MC exhibited a net zero charge at pH 6.66 and 6.81, respectively. Furthermore, the scavenging capabilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide anion radical and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical of ASC-MC and PSC-MC were positively correlated with their tested concentration ranged from 0 to 5 mg/mL and PSC-MC showed significantly higher activity than that of ASC-MC at most tested concentrations ( < 0.05). In addition, the scavenging capability of PSC-MC on hydroxyl radical and superoxide anion radical was higher than those of DPPH radical and ABTS radical, which suggested that ASC-SC and PSC-SC might be served as hydroxyl radical and superoxide anion radical scavenger in cosmeceutical products for protecting skins from photoaging and ultraviolet damage.