Preparation and properties of amperometric uric acid sensors

• Published in: Sensors and actuators. B, Chemical Vol. 52; no. 1-2; pp. 72 - 77
• Main Authors: Kuwabata, Susumu, Nakaminami, Takahiro, Ito, Shin-ichiro, Yoneyama, Hiroshi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.09.1998
• Subjects: Aldehydes; Amino acids; Composition effects; Crosslinking; Drying; Electron mediator; Enzyme immobilization; Gold; Monolayers; Thermal effects; Uric acid sensor; Uricase; Uricase; Electron mediator; Uric acid sensor

Uricase molecules (UOx) were immobilized to a self-assembled monolayer of 2-aminoethanethiolate prepared on an Au electrode substrate by casting phosphate buffer solution containing UOx and glutaraldehyde as a cross-linking agent, followed by drying. The prepared electrode (UOx/Au) exhibited amperom...