Preparation and properties of amperometric uric acid sensors

Uricase molecules (UOx) were immobilized to a self-assembled monolayer of 2-aminoethanethiolate prepared on an Au electrode substrate by casting phosphate buffer solution containing UOx and glutaraldehyde as a cross-linking agent, followed by drying. The prepared electrode (UOx/Au) exhibited amperom...

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Published inSensors and actuators. B, Chemical Vol. 52; no. 1-2; pp. 72 - 77
Main Authors Kuwabata, Susumu, Nakaminami, Takahiro, Ito, Shin-ichiro, Yoneyama, Hiroshi
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.09.1998
Subjects
Aldehydes
Amino acids
Composition effects
Crosslinking
Drying
Electron mediator
Enzyme immobilization
Gold
Monolayers
Thermal effects
Uric acid sensor
Uricase
Uricase
Electron mediator
Uric acid sensor
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Summary:Uricase molecules (UOx) were immobilized to a self-assembled monolayer of 2-aminoethanethiolate prepared on an Au electrode substrate by casting phosphate buffer solution containing UOx and glutaraldehyde as a cross-linking agent, followed by drying. The prepared electrode (UOx/Au) exhibited amperometric responses to uric acid with use of [Fe(CN)6]3− dissolved in solution as an electron mediator at rates much higher than those obtained with use of dissolved UOx. The sensitivity of the UOx/Au electrode was influenced by not only the amount of glutaraldehyde in the casting solution but also the drying time and the temperature after casting the solution. The electrodes prepared under the best preparation conditions showed amperometric responses to uric acid up to 0.6 mmol dm−3, which covers the normal concentration of uric acid in the human blood.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(98)00258-5