Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

• Published in: Marine drugs Vol. 19; no. 5; p. 243
• Main Authors: Abdel-Mageed, Wael M, Al-Wahaibi, Lamya H, Lehri, Burhan, Al-Saleem, Muneera S M, Goodfellow, Michael, Kusuma, Ali B, Nouioui, Imen, Soleh, Hariadi, Pathom-Aree, Wasu, Jaspars, Marcel, Karlyshev, Andrey V
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.04.2021; MDPI
• Subjects: 1H-15N 2D-NMR; Biosynthesis; Deep sea; Deep water; Deferoxamine; desferrioxamine; Environmental conditions; Experiments; Fatty acids; Gene clusters; Genomes; genomics; Glutamine; Mariana Trench; Metabolites; Micromonospora; Micromonospora provocatoris MT25; n-acetylglutaminyl glutamine amide; Natural products; New species; Nitrogen; Nitrogen isotopes; NMR; Nuclear magnetic resonance; Nucleotide sequence; Oceanic trenches; rRNA 16S; Sediments; Sequence analysis; Sequencing; Taxonomy; Mariana Trench; biosynthetic gene clusters; desferrioxamine; 1H-15N 2D-NMR; Mariana Trench; genomics; n-acetylglutaminyl glutamine amide; Micromonospora provocatoris MT25; stress genes
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md19050243

A strain, isolate MT25 , was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, -acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including H- N HSQC and H- N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of , a whole genome sequence analysis revealed it to be most closely related to 45142 . The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25 (NCIMB 15245 , TISTR 2834 ) be classified as sp. nov. Analysis of the genome sequence of strain MT25 (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.