Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination meth...

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Published inJournal of clinical microbiology Vol. 39; no. 6; pp. 2283 - 2286
Main Authors WAGENAAR, Jaap A, VAN BERGEN, Marcel A. P, NEWELL, Diane G, GROGONO-THOMAS, Rose, DUIM, Birgitta
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.06.2001
SeriesNote
Subjects
Animals
Bacterial Typing Techniques
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Campylobacter fetus - classification
Campylobacter fetus - genetics
Campylobacter fetus - metabolism
Clinical Veterinary Microbiology
DNA Fingerprinting - methods
Epidemiology
Fundamental and applied biological sciences. Psychology
Genotype
Microbiology
Phenotype
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Campylobacteraceae
Fragmentation
Infection
Campylobacter infection
Polymerase chain reaction
Fingerprint method
Bacteriosis
Bacteria
Fetus
Isolate
Campylobacter fetus
Differentiation
Comparative study
Polymorphism
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Summary:A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.
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Present address: Department of Clinical Veterinary Science, Division of Animal Health and Husbandry, Bristol, United Kingdom.
Corresponding author. Mailing address: Institute for Animal Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Phone: 31–320-238157. Fax: 31 320–238153. E-mail: j.a.wagenaar@id.wag-ur.nl.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.39.6.2283-2286.2001