MicroRNA-205 suppresses the oral carcinoma oncogenic activity via down-regulation of Axin-2 in KB human oral cancer cell

• Published in: Molecular and cellular biochemistry Vol. 387; no. 1-2; pp. 71 - 79
• Main Authors: Kim, Jae-Sung, Park, Sun-Young, Lee, Seul Ah, Park, Min-Gyeong, Yu, Sun-Kyoung, Lee, Myoung-Hwa, Park, Mi-Ra, Kim, Su-Gwan, Oh, Ji-Su, Lee, Sook-Young, Kim, Chun Sung, Kim, Heung-Joong, Chun, Hong Sung, Kim, Jin-Soo, Moon, Sung-Min, Kim, Do Kyung
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.02.2014; Springer; Springer Nature B.V
• Subjects: 3' Untranslated Regions; Antisense RNA; Apoptosis; Axin Protein - genetics; Axin Protein - metabolism; Base Sequence; Binding Sites; Biochemistry; Biomedical and Life Sciences; Cancer; Carcinoma; Cardiology; Cell Line, Tumor; Cell Survival; Comparative analysis; Gene expression; Gene Expression Regulation, Neoplastic; Health aspects; Humans; Life Sciences; Luciferase; Medical Biochemistry; MicroRNA; MicroRNAs; MicroRNAs - genetics; Mouth cancer; Mouth Neoplasms; Oncology; Oral cancer; RNA Interference; Oral carcinoma; MicroRNA (miRNA); AXIN-2; Oncogenic activity; Apoptosis
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-013-1872-7

MicroRNA (miRNA) is a small noncoding RNA molecule, 19–25 nucleotides in length, which regulates several pathways including cell development, cell proliferation, carcinogenesis, apoptosis, etc. In this study, the over-expression of microRNA-205 (miR-205) increased the number of apoptotic cells by at least 4 times compared to the control. In addition, over-expressed miRNA in KB oral cancer cells triggered apoptosis via the caspase cascade, including the cleavage of caspase-9, caspase-7, caspase-3, and PARP. Flow cytometry showed that apoptotic cell death was increased significantly by 35.33 % in KB oral cancer cells with over-expressed miR-205 compared to the control. The microarray data showed that axis inhibitor protein 2 (Axin2) was down-regulated in KB oral cancer cells transfected with miR-205. In addition, Axin2 was down-regulated by approximately 50 % by over-expressed miR-205 at both the mRNA and protein levels. Interestingly, Axin2 was up-regulated in KB oral cancer compared to human normal oral keratinocytes. Furthermore, the cell cytotoxicity and apoptotic population of KB oral cancer cells were increased significantly after Axin2 siRNA transfection. These results suggest that Axin2 is might be as potential oncogene in KB oral cancer cells. The luciferase assay showed that over-expressed miR-205 in KB oral cancer cells suppressed AXIN2 expression through an interaction with its own binding site at AXIN2 3′UTR (64–92). These results suggest that miR-205 is a novel anti-oncogenic miRNA in KB oral cancer cells, and may have potential applications in oral cancer therapy.