Lack of acquired resistance in dogs to successive infestations of Rhipicephalus sanguineus ticks from Brazil and Argentina

• Published in: Experimental & applied acarology Vol. 67; no. 1; pp. 135 - 146
• Main Authors: Évora, Patricia Martinez, Sanches, Gustavo Seron, Jusi, Márcia Mariza Gomes, Alves, Lucas Bocchini Rodrigues, Machado, Rosangela Zacarias, Bechara, Gervásio Henrique
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2015; Springer Nature B.V
• Subjects: acarology; adults; Animal Ecology; Animal Genetics and Genomics; Animal Systematics/Taxonomy/Biogeography; Animals; antibodies; antigens; Argentina; Biomedical and Life Sciences; Brazil; Comparative studies; cross reaction; Dachshund; dog diseases; Dog Diseases - immunology; Dog Diseases - parasitology; Dogs; Domestic animals; Entomology; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Female; females; hosts; Ixodidae; Life Sciences; Male; males; photoperiod; population; Relative humidity; Reproduction; Rhipicephalus sanguineus; Rhipicephalus sanguineus - physiology; strains; Tick Infestations - veterinary; ticks; titration; Brazil; Argentina; Domestic dogs; Immunity; ELISA
• ISSN: 0168-8162; 1572-9702
• DOI: 10.1007/s10493-015-9936-x

Comparative studies between brown dog tick Rhipicephalus sanguineus populations from Brazil (Jaboticabal, São Paulo) and Argentina (Rafaela, Santa Fé) showed significant biological, morphological and genetic differences between them. This work aimed to study, in a comparative way, the acquisition of resistance in domestic dogs to R. sanguineus from Jaboticabal and Rafaela, after successive and controlled infestations. Ticks were kept in a BOD incubator under controlled conditions (27 °C, 80 % relative humidity, 12-h photoperiod). Ten dogs, Dachshund breed, males and females, 6 months old, short- or long-haired, without prior contact with ticks, were used as hosts. They were distributed into two experimental groups composed of five animals each: G1 infested with ten adult couples of R. sanguineus (Jaboticabal) per animal, and G2 infested with ten adult couples of R. sanguineus (Rafaela) per animal. Ticks’ biological parameters and titration of antibodies from the dogs’ sera by ELISA test were used for comparison between the strains. Results of the biological parameters showed that the dogs did not acquire immunity to either of the R. sanguineus strains after repeated infestations. The ELISA test showed low antibody titers in sera of dogs from G2, in successive infestations, and higher antibody responses post second and third infestations in G1. It also demonstrated cross-reactivity between sera of dogs infested with R. sanguineus (Jaboticabal) and antigens from R. sanguineus (Rafaela) and vice versa. We conclude that Dachshund dogs did not develop resistance against neither Jaboticabal nor Rafaela strains of R. sanguineus.