Effects of Chinese Fructus Mume Formula and Its Separated Prescription Extract on Insulin Resistance in Type 2 Diabetic Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 33; no. 6; pp. 877 - 885
• Main Author: 李井彬 徐丽君 董慧 黄诏宜 赵炎 陈广 陆付耳
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.12.2013; Institute of Integrative Traditional Chinese & Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
• Subjects: 2型糖尿病; Adipose Tissue - drug effects; Adipose Tissue - metabolism; Animals; Arrestins - genetics; Arrestins - metabolism; beta-Arrestin 2; beta-Arrestins; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Glucose Intolerance - drug therapy; Glucose Transporter Type 4 - genetics; Glucose Transporter Type 4 - metabolism; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; Insulin Receptor Substrate Proteins - genetics; Insulin Receptor Substrate Proteins - metabolism; Insulin Resistance; Liver - drug effects; Liver - metabolism; Male; Medicine; Medicine & Public Health; mRNA表达水平; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Rats; Rats, Wistar; Receptor, Insulin - genetics; Receptor, Insulin - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; 乌梅; 公式; 处方; 提取物; 糖尿病大鼠; 胰岛素抵抗; Fructus Mume formula; separated prescription; β-arrestin-2; type 2 diabetes; insulin resistance
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1215-7

The effect of Fructus Mume formula and its separated prescription extract on insulin resis- tance in type 2 diabetic rats was investigated. The rat model of type 2 diabetes was established by feed- ing on a high-fat diet for 8 weeks and by subsequently intravenous injection of small doses of strepto- zotocin. Rats in treatment groups, including the Fructus Mume formula treatment group （FM）, the cold property herbs of Fructus Mume formula treatment group （CFM）, the warm property herbs of Fructus Mume formula treatment group （WFM）, were administrated with Fructus Mume formula and its sepa- rated prescription extract by gavage, while the rats in diabetic model group （DM） and metformin group （MET） were given by gavage with normal saline and metformin correspondingly. The body weight be- fore and after treatment was measured, and the oral glucose tolerance test （OGTT） and the insulin re- lease test （IRT） were performed. The homeostasis model assessment-insulin resistance index （HOMA-IR） was calculated. The protein and mRNA expression levels of Insr, β-arrestin-2, Its-1 and Glut-4 in the liver, skeletal muscle and fat tissues were detected by using Western blotting and RT-PCR respectively. The results demonstrated that, as compared with DM group, OGTT, IRT （0 h, 1 h） levels and HOMR-IR in treatment groups were all reduced, meanwhile their protein and mRNA expression levels of Insr, Irs-1 and Glut-4 in the liver, skeletal muscle and fat tissues were obviously increased, and their protein and mRNA expression levels of β-arrestin-2 in the liver and skeletal muscle tissues were also markedly increased. It was suggested that the Fructus Mume formula and its separated prescription extracts could effectively improve insulin resistance in type 2 diabetic rats, which might be related to the up-regulated expression of Insr, Irs-1 and Glut-4 in the liver, skeletal muscle and fat tissues, and β-arrestin-2 in the liver and skeletal muscle tissues.