Influence of Elicitation and Drying Methods on Anti-Metabolic Syndrome, and Antimicrobial Properties of Extracts and Hydrolysates Obtained from Elicited Lovage ( Levisticum officinale Koch)

• Published in: Nutrients Vol. 13; no. 12; p. 4365
• Main Authors: Jakubczyk, Anna, Złotek, Urszula, Rybczyńska-Tkaczyk, Kamila
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.12.2021; MDPI
• Subjects: alpha-Amylases - antagonists & inhibitors; Anti-Infective Agents; anti-metabolic syndrome; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; antimicrobial properties; Bacteria; Biological activity; Candida albicans - drug effects; Convection; Cyclopentanes; Desiccation - methods; Drug Resistance, Fungal; drying method; elicitors; Enzyme Inhibitors; Enzymes; Ethanol; Food; Freeze drying; Glucosidase; Glycoside Hydrolase Inhibitors; Hydrolysates; Hydrolysis; Influence; Jasmonic acid; Leaves; Levisticum - chemistry; Levisticum officinale; lovage; Metabolic disorders; Metabolic syndrome; Metabolites; Methods; Microwaves; Minimum inhibitory concentration; Oxylipins; Plant Extracts - pharmacology; Plant resistance; Solid Phase Extraction - methods; Statistical analysis; Yeast; Yeasts; α-Amylase; α-Glucosidase; anti-metabolic syndrome; antimicrobial properties; lovage; drying method; elicitors
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13124365

This research aims to investigate the influence of elicitation and drying methods (natural, convection, microwave, and freeze-drying), with jasmonic acid (JA) and yeast extract (YE) on the biological activity of extracts and hydrolysates from lovage ( Koch) leaves. The results indicate that the highest TPC was determined for hydrolysates obtained from JA-elicited microwave-dried lovage (24.96 mg/gDW). The highest ACE and lipase inhibitory activity was noted for PBS extract obtained from JA-elicited lovage after microwave drying (EC = 0.16 and 0.12 mg/mL, respectively). Ethanolic extract from JA-elicited lovage after freeze-drying was characterized by the highest α-amylase inhibitory activity (EC = 3.92 mg/mL) and the highest α-glucosidase inhibitory activity (EC = 1.43 mg/mL) was noted for hydrolysates from control plants subjected to freeze-drying. The highest antimicrobial activity towards yeasts was observed for microwave ethanolic extracts with minimal inhibition (MIC) and lethal (MLC) concentrations of 0.625 and 1.25 mg/mL, respectively.