Expression of type VI adenylyl cyclase in the central nervous system: implication for a potential regulator of multiple signals in different neurotransmitter systems

• Published in: FEBS letters Vol. 436; no. 1; pp. 92 - 98
• Main Authors: Liu, Fu-Chin, Wu, Gour-Cheng, Hsieh, Sung-Tsang, Lai, Hsing-Lin, Wang, Hsiao-Fang, Wang, Tsu-Wei, Chern, Yijuang
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 25.09.1998
• Subjects: Adenylyl cyclase; Adenylyl Cyclases - genetics; Adenylyl Cyclases - immunology; Adenylyl Cyclases - metabolism; Animals; Brain; Brain - enzymology; Calcium; Calcium - metabolism; Calcium - pharmacology; cAMP; Central Nervous System - enzymology; Immune Sera; Immunocytochemistry; Immunohistochemistry - methods; Microtubule-Associated Proteins - metabolism; Neurons - enzymology; Neurotransmitter Agents - metabolism; Polymerase Chain Reaction; Protein kinase C; Rabbits; Rats; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Transcription, Genetic; Tyrosine 3-Monooxygenase - metabolism; Brain; Protein kinase C; Adenylyl cyclase; Calcium; cAMP; Immunocytochemistry
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)01098-9

The aim of this study was to investigate the distribution of a calcium-inhibitable adenylyl cyclase type VI (type VI AC) in the central nervous system using an antiserum directed against the N-terminus of type VI AC. Our results indicate that type VI AC immunoreactivity is generally expressed in many brain regions with different levels of intensity. Most interestingly, the majority of the detected type VI AC immunoreactivity is present in cells of neuronal phenotype. Double immunostaining of type VI AC and markers of various neurotransmitter systems suggest that type VI AC might participate in regulation of the classical neurotransmitter systems and therefore appeared to play a very important role in the central nervous system.