Formation of new lymphatic vessels in glioma: An immunohistochemical analysis

• Published in: Neuropathology Vol. 40; no. 3; pp. 215 - 223
• Main Authors: Meng, Fan‐Wei, Liu, Fu‐Sheng, Liu, Wen‐Hui, Li, Li, Jie, Lin‐Lin
• Format: Journal Article
• Language: English
• Published: Melbourne John Wiley & Sons Australia, Ltd 01.06.2020; Wiley Subscription Services, Inc
• Subjects: Adolescent; Adult; Biomarkers, Tumor - analysis; Blood vessels; Brain Neoplasms - pathology; CD34 antigen; Cell differentiation; Endothelial cells; Female; Glioma; Glioma - pathology; Glioma cells; Homeobox; Humans; Hyaluronic acid; Hypoxia; hypoxia‐inducible factor 1α; Immunohistochemistry; lymphatic endothelial hyaluronic acid receptor 1; Lymphatic system; lymphatic vessels; Lymphatic Vessels - pathology; Male; Middle Aged; Nestin; Original; Prospero protein; Prospero‐Related Homeobox 1; Stem cells; Young Adult; hypoxia-inducible factor 1α; lymphatic vessels; Prospero-Related Homeobox 1; glioma; lymphatic endothelial hyaluronic acid receptor 1
• ISSN: 0919-6544; 1440-1789
• DOI: 10.1111/neup.12625

We investigated the distribution and formation of new lymphatic vessels in gliomas. Specimens from seven glioma cases were analyzed by immunohistochemical staining for CD34, lymphatic endothelial hyaluronic acid receptor 1 (LYVE‐1), prospero‐related homeobox 1 (Prox1), nestin, and hypoxia‐inducible factor 1α (HIF‐1α). Three types of vessels were observed in glioma specimens: LYVE‐1+ lymphatic vessels, CD34+ blood vessels, and LYVE‐1+/CD34+ blood vessels. Prox1+/LYVE‐1+ cells were distributed in some lymphatic vessels as well as among vascular endothelial cells and glioma cells. Nestin+ cells were scattered throughout the gliomas, and some lymphatic cells also expressed nestin. HIF‐1α+ Prox1+ cells were widely distributed within the glioma specimens. The present immunohistochemical analysis revealed upregulation of Prox1 and HIF‐1α in some glioma tissues as well as the differentiation of nestin+ tumor stem cells into LYVE‐1+ lymphatic vessels.