Structure of a fluid dioleoylphosphatidylcholine bilayer determined by joint refinement of x-ray and neutron diffraction data. I. Scaling of neutron data and the distributions of double bonds and water

• Published in: Biophysical journal Vol. 60; no. 3; pp. 568 - 576
• Main Authors: Wiener, M.C., King, G.I., White, S.H.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.09.1991; Biophysical Society; The Biophysical Society
• Subjects: Artificial membranes and reconstituted systems; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Lipid Bilayers; Membrane physicochemistry; Molecular biophysics; Molecular Conformation; Neutrons; Phosphatidylcholines - chemistry; Scattering, Radiation; X-Ray Diffraction; Molecular orientation; Neutron diffraction; Liposome; Phosphatidylcholine; Bilayer; X ray diffraction; Double bond
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(91)82086-0

We described in two previous papers a method for the joint refinement of the structure of fluid bilayers using neutron and x-ray diffraction data (Wiener, M. C., and S. H. White 1991a, b. Biophys. J. 59: 162–173 and 174–185). An essential part of the method is the appropriate scaling of the diffraction data. Here we describe the scaling of the neutron data and the determination of the transbilayer distribution of double bonds in liquid-crystalline (L alpha phase) phospholipid bilayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The distribution was determined by neutron diffraction of oriented multilayers (66% RH) of DOPC specifically deuterated at the 9- and 10-position of both acyl chains. The double-bond distribution is described accurately by a pair of Gaussian functions each located at a position Zcc = 7.88 +/- 0.09 A from the bilayer center with 1/e-halfwidths of Acc = 4.29 +/- 0.16 A. Previously, we determined the transbilayer distribution of bromine atoms in a specifically halogenated lipid, 1-oleoyl-2–9,10-dibromostearoyl-sn-glycero-3-phosphocholine (OBPC), and showed it to be an isomorphous replacement for DOPC (Wiener, M. C., and S. H. White, 1991c. Biochemistry. In press). A comparison of the double-bond and bromine profiles indicates that the positions of the centers of the deuterated double bond and the brominated methylene Gaussian distributions are equal within experimental error and that each label undergoes similar average thermal motions with respect to the bilayer normal.