High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China

• Published in: Soil biology & biochemistry Vol. 70; pp. 113 - 122
• Main Authors: Liu, Junjie, Sui, Yueyu, Yu, Zhenhua, Shi, Yu, Chu, Haiyan, Jin, Jian, Liu, Xiaobing, Wang, Guanghua
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01.03.2014; Elsevier
• Subjects: Acidobacteria; Actinobacteria; Agronomy. Soil science and plant productions; bacterial communities; Bacterial diversity; Biochemistry and biology; biogeography; Biological and medical sciences; carbon; Chemical, physicochemical, biochemical and biological properties; China; edaphic factors; food security; Fundamental and applied biological sciences. Psychology; high-throughput nucleotide sequencing; latitude; Latitudinal diversity gradient; Microbiology; Mollisols; nucleotide sequences; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Planctomycetes; Proteobacteria; ribosomal DNA; soil bacteria; Soil carbon content; Soil microbial biomass; Soil pH; soil resources; soil sampling; Soil science; variance; Asia; China; China, People's Rep; Soil pH; Latitudinal diversity gradient; Mollisols; Bacterial diversity; Soil microbial biomass; Soil carbon content; Gradient; Biogeography; Property of soil; Latitude; Biodiversity; Microbial biomass; Carbon content; Geographic distribution; pH; Bacteria; Soil science; Sequencing; Microbial community
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/j.soilbio.2013.12.014

Black soils (Mollisols) are one of the most important soil resources for maintaining food security of China and are mainly distributed in northeast China. To understand which environmental factors influence the microbial communities and how the communities are distributed in the black soils, we collected 26 soil samples with different soil carbon contents across the black soil zone in northeast China, and the soil bacterial community compositions were estimated using high resolution bar-coded pyrosequencing. A total of 355,813 bacterial 16S rDNA sequences were obtained, which were classified into at least 35 bacterial groups. The dominant groups across all samples (>5% of all sequences) were Acidobacteria, Actinobacteria, Proteobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes and Planctomycetes. The composition and diversity of the soil bacterial community were dominantly affected by both soil pH and soil total carbon content, and the effect of soil pH was stronger than that of soil carbon content. Variance partitioning analysis showed that geographic distance contributed 14.75% of the bacterial community variation, and soil environmental factors explained approximately 37.52% of the variation. Pairwise analysis showed that a relatively higher diversity of the bacterial community was observed at lower latitudes, suggesting that a latitudinal diversity gradient of the bacterial community might be present in the black soil zone. In general, our results indicated that contemporary factors, such as soil pH and soil carbon content, were more important than the historical factor of geographic distance in shaping the bacterial community in the black soil zone in northeast China. Variation partition analysis of the effects of geographic distance and soil variables on the phylogenetic structure of bacterial communities in the black soils of northeast China. [Display omitted] •Bacterial communities in Mollisols were both affected by soil pH and soil C content.•The effect of soil pH on bacterial communities was stronger than soil C content.•Geographic distance was another important factor in shaping communities.•A latitudinal diversity gradient of the community was observed in the Mollisols.•Soil bacterial communities were spatially distributed in the black soil zone.