Casein materials show different digestion patterns using an in vitro gastrointestinal model and different release of glucagon-like peptide-1 by enteroendocrine GLUTag cells

• Published in: Food chemistry Vol. 277; pp. 423 - 431
• Main Authors: Komatsu, Yosuke, Wada, Yasuaki, Izumi, Hirohisa, Shimizu, Takashi, Takeda, Yasuhiro, Hira, Tohru, Hara, Hiroshi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.03.2019
• Subjects: Animals; Bioactive peptide; Casein material; Caseins - chemistry; Cell Line; Chemical Phenomena; Chromatography, Liquid; Digestion; Enteroendocrine Cells - metabolism; Gastrointestinal Tract - metabolism; Glucagon-Like Peptide 1 - metabolism; Glucagon-like peptide-1; GLUTag cells; In vitro gastrointestinal digestion; Manufacturing process; Mice; Models, Biological; Molecular Weight; Particle Size; Peptidomics; Protein digestibility; Solid Phase Extraction; Tandem Mass Spectrometry; Glucagon-like peptide-1; Protein digestibility; Manufacturing process; Casein material; In vitro gastrointestinal digestion; Bioactive peptide; GLUTag cells; Peptidomics
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2018.10.123

•Properties of casein materials depend on the production process.•Micellar casein concentrate has higher digestibility than sodium caseinate.•Both casein materials showed different peptide patterns after in vitro digestion.•Micellar casein concentrate strongly induced glucagon-like peptide-1 release.•GPVRGPFPIIV peptide derived from β-casein induced glucagon-like peptide-1 release. Physicochemical properties of casein (CN) materials manufactured using different processes are well studied; however, data on their bioaccessibility or bioactivity are limited. We compared the digestion patterns and glucagon-like peptide-1 (GLP-1)–releasing activities of micellar CN concentrate (MCC) and sodium caseinate (SCN). MCC and SCN mixed with whey protein isolate (SCN + WPI) were subjected to in vitro gastrointestinal digestion; the digestibility of MCC was higher than that of SCN + WPI, and both CN materials showed different patterns of peptides released after in vitro digestion. A comparison of GLP-1–releasing activities showed that MCC induced GLP-1 secretion to a greater extent than SCN + WPI. Candidate peptides identified from CN digesta were chemically synthesized to test their GLP-1–releasing activity. GPVRGPFPIIV identified only in the MCC digesta, could stimulate GLP-1 release. In conclusion, the digestion patterns and GLP-1–releasing activity of CN materials depend on the production process.