Phlebotomus papatasi and Meriones libycus as the vector and reservoir host of cutaneous leishmaniasis in Qomrood District,Qom Province,central Iran
<正>Objective:To determine the sand flies species responsible for most transmission of Leishmania major(L major) to human,as well as to determine the main reservoir hosts of the disease. Methods:Sand flies were collected using sticky papers and mounted in Puri’s medium for species ident...
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Published in | Asian Pacific journal of tropical medicine Vol. 4; no. 2; pp. 97 - 100 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
India
Elsevier B.V
01.02.2011
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Subjects | |
Online Access | Get full text |
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Summary: | <正>Objective:To determine the sand flies species responsible for most transmission of Leishmania major(L major) to human,as well as to determine the main reservoir hosts of the disease. Methods:Sand flies were collected using sticky papers and mounted in Puri’s medium for species identification.Rodents were trapped by live Sherman traps.Both sand flies and rodents were subjected to molecular methods for detection of leishmanial parasite.Results:Phlebotomus papatasi(P.papatasi) was the common species in outdoor and indoor resting places.Employing PCR technique only three specimens of 150 P.papatasi(2%) were found naturally infected by parasites with a band of 350 bp which is equal to the L major parasite.Forty six rodents were captured by Sherman traps and identified.Microscopic investigation on blood smear of the animals for amastigote parasites revealed 1(3.22%) infected Meriones libycus(M.libycus). Infection of this animal to L.major was confirmed by PCR against rDNA loci of the parasite. Conclusions:This is the first molecular report of parasite infection of both vector(P.papatasi) and reservoir(M.libycus) to L major in the region.The results indicated that P.papatasi was the primary vector of the disease and circulating the parasite between human and reservoirs and M. libycus was the most important host reservoir for maintenance of the parasite source in the area. |
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Bibliography: | Rassi Yavar~(1*),Saghafipour Abedin~1,Abai Mohammad Reza~1,Oshaghi Mohammad Ali~1,Rafizadeh Sina~2, Mohebail Mehdi~3,Yaaghobi-Ershadi Mohammad Reza~1,Mohtarami Fatemeh~1,Farzinnia Babak~4 1 Department of Medical Entomology and Vector Control,School of Public Health,Tehran University of Medical Sciences,Iran 2 Department of Emergency,Ministry of Health and Medical Education,Iran 3 Department of Parasitology and Mycology,School of Public Health,Tehran University of Medical Sciences,Iran 4 Healh Center of Qom,Qom Province,Iran ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1995-7645 2352-4146 |
DOI: | 10.1016/S1995-7645(11)60045-X |