Effect of Rhaphidophora korthalsii methanol extract on human peripheral blood mononuclear cell (PBMC) proliferation and cytolytic activity toward HepG2

• Published in: Journal of ethnopharmacology Vol. 114; no. 3; pp. 406 - 411
• Main Authors: Yeap, S.K., Alitheen, N.B., Ali, A.M., Omar, A.R., Raha, A.R., Suraini, A.A., Muhajir, A.H.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 03.12.2007; Elsevier
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Araceae; Biological and medical sciences; carcinoma; cell cycle; Cell Line, Tumor; cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Cytotoxic; cytotoxicity; Cytotoxicity, Immunologic - drug effects; flow cytometry; General pharmacology; hepatocytes; HepG2; human cell lines; Humans; immune system; Immunomodulator; immunostimulants; in vitro studies; Killer Cells, Natural - immunology; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - immunology; Medical sciences; medicinal plants; medicinal properties; monocytes; PBMC; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; plant extracts; Plant Extracts - pharmacology; Rhaphidophora; Rhaphidophora korthalsii; Immunomodulator; HepG2; Rhaphidophora korthalsii; Cytotoxic; PBMC; Human; Cell proliferation; Biological fluid; Pharmacognosy; Cytotoxicity; Extract; Blood; Medicinal plant; Mononuclear cell; Plant origin
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.08.020

The study of bioactivity of natural product is one of the major researches for drug discovery. The aim of this finding was to study the proliferation effect of Rhaphidophora korthalsii methanol extract on human PBMC and subsequently the cytotoxic effect of activated PBMC toward HepG2 human hepatocellular carcinoma. In this present study, MTT assay, cell cycle study and Annexin 5 binding assay were used to study the immunomodulatory and cytotoxic effects. In vitro cytotoxic screening of Rhaphidophora korthalsii methanol extract showed that the extract was non-toxic against hepatocellular carcinoma (HepG2). In contrast, the extract was able to stimulate the proliferation of human PBMC at 48 h and 72 h in MTT assay and cell cycle progress study. The application of immunomodulator in tumor research was studied by using MTT microcytotoxicity assay and flow cytometric Annexin V. Results indicated that pre-treated PBMC with Rhaphidophora korthalsii methanol extract induced the highest cytotoxicity (44.87 ± 6.06% for MTT microcytotoxicity assay and 51.51 ± 3.85% for Annexin V) toward HepG2. This finding demonstrates that Rhaphidophora korthalsii methanol extract are potent to stimulate the cytotoxic effect of immune cells toward HepG2.