In vitro and in vivo antioxidant properties and DNA damage protective activity of green fruit of Ficus glomerata

• Published in: Food and chemical toxicology Vol. 48; no. 2; pp. 704 - 709
• Main Authors: Verma, Arti R., Vijayakumar, M., Rao, Chandana V., Mathela, Chandra S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.02.2010; Elsevier
• Subjects: animal models; Animals; antioxidant activity; Antioxidants; Biocompatibility; Biological and medical sciences; Biomedical materials; Carbon Tetrachloride Poisoning - enzymology; Carbon Tetrachloride Poisoning - prevention & control; Damage; DNA Breaks, Single-Stranded - drug effects; DNA damage; DNA repair; Ficus; Ficus - chemistry; Ficus glomerata; Ficus racemosa; Food Analysis; free radical scavengers; Free Radical Scavengers - analysis; Free Radical Scavengers - pharmacology; Fruit - chemistry; fruit extracts; Glutathione - metabolism; In vitro and in vivo antioxidant properties; in vitro studies; In Vitro Techniques; In vitro testing; in vivo studies; In vivo testing; In vivo tests; Lipid Peroxidation - drug effects; Medical sciences; Oxidative DNA damage; oxidative stress; Oxidative Stress - drug effects; Oxidoreductases - metabolism; phenolic compounds; Phenolic content and HPLC; Phenols - analysis; plant extracts; Plant Extracts - pharmacology; Plant poisons toxicology; Rats; Rats, Sprague-Dawley; Surgical implants; Toxicology; tree fruits; IC 50; Phenolic content and HPLC; TPC; HRS; FDF; LPO; MDA; AOA; ASE; CMC; GSH; HPLC; Ficus glomerata; DPPH; FEF; FCF; FCE; SARS; SOD; FAF; GAE; Oxidative DNA damage; In vitro and in vivo antioxidant properties; EC 50; CAT; ROS; RP; ARP; Oxidative stress; HPLC chromatography; Prevention; Dicotyledones; Angiospermae; Phenoplasts; Fruit; Nutrition; Moraceae; Woody plant; Antioxidant; In vitro; Biological activity; Feeding; In vivo; Diet; Ficus; Content; Plant origin; Spermatophyta; Lesion; Green
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2009.11.052

This study evaluated the antioxidant properties of Ficus glomerata fruits using in vitro and in vivo assay. In order to find in vitro antioxidant properties, extract/fractions from F. glomerata were studied for TPC, AOA, RP, DPPH , O 2 - , OH scavenging activities and LPO. Among all the extract/fractions, FEF has shown potent antioxidant activity and was also found effective in protecting oxidative DNA damage. The in vivo evaluation of oxidative stress (LPO) and antioxidant defenses (concentration of GSH, as well as CAT and SOD activities) were measured in CCl 4 induced toxic rats. FEF was found to inhibit the toxicity as seen from the decreased LPO and increased GSH, SOD and CAT levels. FEF has higher phenolic content and showed the presence of gallic, chlorogenic and ellagic acid. Based on these results, it is concluded that F. glomerata protects tissues from oxidative stress and these effects are probably related to the antioxidant properties.