Analysis of Outer Membrane Vesicles Indicates That Glycerophospholipid Metabolism Contributes to Early Symbiosis Between Sinorhizobium fredii HH103 and Soybean

• Published in: Molecular plant-microbe interactions Vol. 35; no. 4; pp. 311 - 322
• Main Authors: Li, Dongzhi, Li, Ziqi, Wu, Jing, Tang, Zhide, Xie, Fuli, Chen, Dasong, Lin, Hui, Li, Youguo
• Format: Journal Article
• Language: English
• Published: United States American Phytopathological Society 01.04.2022; The American Phytopathological Society
• Subjects: Animals; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biosynthesis; Correlation analysis; deformation; Differential thermal analysis; early symbiosis; Encyclopedias; Enzyme-linked immunosorbent assay; Fabaceae - microbiology; Flagella; Gene expression; Genes; Genistein; Genomes; glycerophospholipids; Glycerophospholipids - metabolism; Glycine max - microbiology; Gram-negative bacteria; Lipid metabolism; lipidomics; Lipids; Living conditions; Mammals - metabolism; Membrane vesicles; Membranes; Metabolic pathways; Metabolism; Mimicry; Nitrogen metabolism; Nodulation; outer membrane vesicles; Oxidative metabolism; Oxidative phosphorylation; Peptidoglycans; Phosphatidylserine; Phosphatidylserine decarboxylase; phosphatidylserines; Phosphorylation; proteome; Proteomes; Proteomics; Quorum sensing; rhizobia; Rhizobium; ribosomes; Root hairs; Sinorhizobium fredii; Sinorhizobium fredii - genetics; Soybeans; Symbiosis; Symbiosis - genetics; Vesicles; correlation analysis; early symbiosis; outer membrane vesicles; proteomics; rhizobia; lipidomics
• ISSN: 0894-0282; 1943-7706; 1943-7706
• DOI: 10.1094/MPMI-11-21-0288-R

Gram-negative bacteria can produce outer membrane vesicles (OMVs), and most functional studies of OMVs have been focused on mammalian-bacterial interactions. However, research on the OMVs of rhizobia is still limited. In this work, we isolated and purified OMVs from Sinorhizobium fredii HH103 under free-living conditions that were set as control (C-OMVs) and symbiosis-mimicking conditions that were induced by genistein (G-OMVs). The soybean roots treated with G-OMVs displayed significant deformation of root hairs. G-OMVs significantly induced the expression of nodulation genes related to early symbiosis, while they inhibited that of the defense genes of soybean. Proteomics analysis identified a total of 93 differential proteins between C-OMVs and G-OMVs, which are mainly associated with ribosome synthesis, flagellar assembly, two-component system, ABC transporters, oxidative phosphorylation, nitrogen metabolism, quorum sensing, glycerophospholipid metabolism, and peptidoglycan biosynthesis. A total of 45 differential lipids were identified through lipidomics analysis. Correlation analysis of OMV proteome and lipidome data revealed that glycerophospholipid metabolism is the enriched Kyoto Encyclopedia of Genes and Genomes metabolic pathway, and the expression of phosphatidylserine decarboxylase was significantly up-regulated in G-OMVs. The changes in three lipids related to symbiosis in the glycerophospholipid metabolism pathway were verified by enzyme-linked immunosorbent assay. Our results indicate that glycerophospholipid metabolism contributes to rhizobia-soybean symbiosis via OMVs. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .