Evaluation of BMPA, MWY, GVPC and BCYE media for the isolation of Legionella species from respiratory samples

Culture media performance is a critical factor in the isolation of Legionellae from respiratory samples. We showed that BMPA and MWY media yielded significantly higher isolation rates than GVPC and BCYE media in regard to performance with samples that harbored low Legionella inocula and high contami...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 98; pp. 119 - 121
Main Authors Descours, G., Cassier, P., Forey, F., Ginevra, C., Etienne, J., Lina, G., Jarraud, S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.03.2014
Elsevier
Subjects
BMPA
Culture Media - metabolism
GVPC
Humans
Isolation
Legionella
Legionella - isolation & purification
Life Sciences
MWY
Respiratory samples
Respiratory System - microbiology
Respiratory samples
Isolation
Legionella
BMPA
GVPC
MWY
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Summary:Culture media performance is a critical factor in the isolation of Legionellae from respiratory samples. We showed that BMPA and MWY media yielded significantly higher isolation rates than GVPC and BCYE media in regard to performance with samples that harbored low Legionella inocula and high contamination levels. •BMPA & MWY are the most sensitive plates for Legionella isolation from human samples.•BMPA & MWY are also the least contaminated plates.•The use of a combination of GVPC and BCYE plates is not recommended.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2014.01.001