Genetic differences between Tunisian camel and sheep strains of the cestode Echinococcus granulosus revealed by SSCP

• Published in: Parasite (Paris) Vol. 13; no. 2; pp. 131 - 136
• Main Authors: Oudni-M’rad, M., Cabaret, J., M’rad, S., Bouzid, W., Mekki, M., Belguith, M., Sayadi, T., Nouri, A., Lahmar, S., Azaiez, R., Mezhoud, H., Babba, H.
• Format: Journal Article
• Language: English
• Published: Paris EDP Sciences 01.06.2006; Princeps
• Subjects: Animals; Biological and medical sciences; Camelus - parasitology; Echinococcosis - parasitology; Echinococcosis - veterinary; Echinococcus granulosus; Echinococcus granulosus - classification; Echinococcus granulosus - genetics; Electron Transport Complex IV - genetics; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genetics of eukaryotes. Biological and molecular evolution; Host-Parasite Interactions; hydatidose; hydatidosis; Invertebrata; Life Sciences; lignée; Phylogeny; Polymorphism, Single-Stranded Conformational; polymorphisme de conformation simple brin; Population genetics, reproduction patterns; Sheep; Sheep Diseases - parasitology; Species Specificity; SSCP; strain; Tunisia; Tunisie; variabilité génétique; Tunisia; Africa; Host parasite relation; Genetic variability; Enzyme; Camelus dromedarius; Cytochrome-c oxidase; Plathelmintha; Parasite; Cestoda; Population genetics; Single strand conformation polymorphism; Vertebrata; Mammalia; Ovis aries; Pathogenic; Helmintha; Host range; Artiodactyla; Oxidoreductases; Intraspecific comparison; Invertebrata; Animal population; Ungulata; Echinococcus granulosus; strain; genetic variation; sscp; echinococcus granulosus; tunisia; hydatidosis
• ISSN: 1252-607X; 1776-1042
• DOI: 10.1051/parasite/2006132131

Ovine and dromedary Echinococcus granulosus isolates from Tunisia were identified as G1 and G6 strains based on polymorphism of the mitochondrial cytochrome C oxydase CO1. Single strand conformation polymorphism (SSCP) was used in order to examine the genetic variation within and between Tunisian G1 and G6 strains and to estimate the extent of selfing. The dromedary isolates are genetically distinct from sheep isolates (high value of genetic variation between populations: Fst = 0.46). No significant deficiency in heterozygotes was found in sheep isolates, whereas heterozygote deficiency (suggesting selfing) was found in a limited number of camel isolates. Les Echinococcus granulosus de Tunisie ont été identifiés comme appartenant aux lignées G1 et G6 en se fondant sur le polymorphisme de la cytochrome oxydase CO1. Le polymorphisme de conformation simple brin (SSCP) a été utilisé afin d’évaluer la variabilité génétique intra et inter-isolats des lignées G1 et G6, et pour estimer le taux d’autofécondation. Les isolats issus de dromadaires sont génétiquement distincts des isolats ovins (valeur de Fst : 0,46). Il n’y a pas de déficit significatif en hétérozygotes chez les isolats ovins. Un déficit en hétérozygotes est présent dans les isolats du dromadaire, ce qui suggère l’autofécondation comme l’un des modes de reproduction.