Rutin from Dendropanax morbifera Leveille Protects Human Dopaminergic Cells Against Rotenone Induced Cell Injury Through Inhibiting JNK and p38 MAPK Signaling

• Published in: Neurochemical research Vol. 39; no. 4; pp. 707 - 718
• Main Authors: Park, Se-Eun, Sapkota, Kumar, Choi, Jun-Hui, Kim, Myung-Kon, Kim, Young Hoi, Kim, Ki Man, Kim, Kyung Je, Oh, Ha-Na, Kim, Sung-Jun, Kim, Seung
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.04.2014; Springer Nature B.V
• Subjects: Araliaceae; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Line, Tumor; Cell Survival - drug effects; Cell Survival - physiology; Dendropanax; Dopaminergic Neurons - drug effects; Dopaminergic Neurons - enzymology; Dose-Response Relationship, Drug; Humans; JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors; JNK Mitogen-Activated Protein Kinases - metabolism; Neurochemistry; Neurology; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; Neurosciences; Original Paper; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases - metabolism; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Protein Kinase Inhibitors - isolation & purification; Protein Kinase Inhibitors - pharmacology; Rotenone - antagonists & inhibitors; Rotenone - toxicity; Rutin - isolation & purification; Rutin - pharmacology; Rutin; SH-SY5Y cells; Parkinson’s disease; Leveille; Rotenone
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-014-1259-5

Dendropanax morbifera Leveille (Araliaceae) is well known in Korean traditional medicine for a variety of diseases. Rotenone is a commonly used neurotoxin to produce in vivo and in vitro Parkinson’s disease models. This study was designed to elucidate the processes underlying neuroprotection of rutin, a bioflavonoid isolated from D. morbifera Leveille in cellular models of rotenone-induced toxicity. We found that rutin significantly decreased rotenone-induced generation of reactive oxygen species levels in SH-SY5Y cells. Rutin protected the increased level of intracellular Ca 2+ and depleted level of mitochondrial membrane potential (ΔΨm) induced by rotenone. Furthermore, it prevented the decreased ratio of Bax/Bcl-2 caused by rotenone treatment. Additionally, rutin protected SH-SY5Y cells from rotenone-induced caspase-9 and caspase-3 activation and apoptotic cell death. We also observed that rutin repressed rotenone-induced c-Jun N-terminal kinase and p38 mitogen-activated protein kinase phosphorylation. These results suggest that rutin may have therapeutic potential for the treatment of neurodegenerative diseases associated with oxidative stress.