Molecular and immunological analyses of the Borrelia turicatae Bdr protein family

Here, we describe the molecular and immunological characterization of the bdr gene family of Borrelia turicatae, a relapsing-fever spirochete. Nine bdr alleles belonging to two different subfamilies were sequenced and localized to linear plasmids. Anti-Bdr antiserum was generated and used to analyze...

Full description

Saved in:
Bibliographic Details
Published inInfection and immunity Vol. 68; no. 4; pp. 2369 - 2373
Main Authors CARLYON, J. A, ROBERTS, D. M, THEISEN, M, SADLER, C, MARCONI, R. T
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.04.2000
SeriesNote
Subjects
Alleles
Amino Acid Sequence
Animals
Bacterial Proteins - chemistry
Bacteriology
bdr gene
Bdr protein
Biological and medical sciences
Borrelia - genetics
Borrelia turicatae
Escherichia coli - metabolism
Evolution, Molecular
Fundamental and applied biological sciences. Psychology
Gene Library
Genetics
Immunoblotting
Male
Mice
Mice, Inbred C3H
Microbiology
Molecular and Cellular Pathogenesis
Molecular Sequence Data
Multigene Family
Oligonucleotide Probes
Open Reading Frames
Plasmids
Sequence Homology, Amino Acid
Temperature
Allele
Spirochaetaceae
Spirochaetales
Gene
Sequence alignment
Bacteria
Borrelia turicatae
Multigene family
Comparative study
Structural analysis
Online AccessGet full text

Cover

More Information
Summary:Here, we describe the molecular and immunological characterization of the bdr gene family of Borrelia turicatae, a relapsing-fever spirochete. Nine bdr alleles belonging to two different subfamilies were sequenced and localized to linear plasmids. Anti-Bdr antiserum was generated and used to analyze Bdr expression in pre- and postinfection isogenic populations. The analyses presented here provide a detailed characterization of the Bdr proteins in a relapsing-fever spirochete species, enhancing our understanding of these proteins at the genus-wide level.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298. Phone: (804) 828-3779. Fax: (804) 828-9946. E-mail: RMARCONI@hsc.vcu.edu.
Present address: Department of Internal Medicine, Section of Rheumatology, Laboratory of Clinical Investigation 604, Yale University, New Haven, CT 06520-8031.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.68.4.2369-2373.2000