Irisin Contributes to the Hepatoprotection of Dexmedetomidine during Intestinal Ischemia/Reperfusion

• Published in: Oxidative medicine and cellular longevity Vol. 2019; no. 2019; pp. 1 - 15
• Main Authors: Zhou, Jun, Zhang, Chunxiang, Chen, Ye, Yang, Bo, Li, Jia-Man, Wang, Mao-Hua, Du, Juan, Fan, Xin, Dai, Jun-Chao
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2019; Hindawi; Hindawi Limited
• Subjects: Alanine Transaminase - metabolism; Animals; Antibodies, Neutralizing - therapeutic use; Apoptosis; Aspartate Aminotransferases - metabolism; Atherosclerosis; Brain research; Dexmedetomidine - therapeutic use; Diabetes; Experiments; Fibronectins - antagonists & inhibitors; Fibronectins - blood; Fibronectins - metabolism; Inflammatory diseases; Intestines - drug effects; Intestines - pathology; Ischemia; Ischemia - blood; Ischemia - drug therapy; Laboratory animals; Liver; Liver - drug effects; Liver - metabolism; Male; Malondialdehyde - metabolism; Medical research; Metabolism; Musculoskeletal system; Oxidative stress; Peptides; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Rats, Sprague-Dawley; Reperfusion Injury - blood; Reperfusion Injury - drug therapy; Rodents; Systematic review; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2019/7857082

Intestinal ischemia/reperfusion (I/R), which is associated with high morbidity and mortality, is also accompanied with abnormal energy metabolism and liver injury. Irisin, a novel exercise-induced hormone, can regulate adipose browning and thermogenesis. The following study investigated the potential role of dexmedetomidine in liver injury during intestinal I/R in rats. Adult male Sprague–Dawley rats underwent occlusion of the superior mesenteric artery for 90 min followed by 2 h of reperfusion. Dexmedetomidine or irisin-neutralizing antibody was intravenously administered for 1 h before surgery. The results demonstrated that severe intestine and liver injuries occurred during intestinal I/R as evidenced by pathological scores and an apparent increase in serum diamine oxidase (DAO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels. In addition, the hepatic irisin, cleaved caspase-3, Bax, and NLRP3 inflammasome components (including NLRP3, ASC, and caspase-1), protein expressions, apoptotic index, reactive oxygen species (ROS), malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor- (TNF-) α, and interleukin- (IL-) 6 levels increased; however, the serum irisin level and hepatic Bcl-2 protein expression and superoxide dismutase (SOD) activity decreased after intestinal I/R. Interestingly, dexmedetomidine could reduce the above listed changes and increase the irisin levels in plasma and the liver in I/R rats. Dexmedetomidine-mediated protective effects on liver injury and NLRP3 inflammasome activation during intestinal I/R were partially abrogated via irisin-neutralizing antibody treatment. The results suggest that irisin might contribute to the hepatoprotection of dexmedetomidine during intestinal ischemia/reperfusion.