Harnessing the synergy of perfusable muscle flap matrix and adipose-derived stem cells for prevascularization and macrophage polarization to reconstruct volumetric muscle loss

• Published in: Bioactive materials Vol. 22; pp. 588 - 614
• Main Authors: Zhang, Qixu, Chiu, Yulun, Chen, Youbai, Wu, Yewen, Dunne, Lina W., Largo, Rene D., Chang, Edward I., Adelman, David M., Schaverien, Mark V., Butler, Charles E.
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.04.2023; KeAi Publishing; KeAi Communications Co., Ltd
• Subjects: Decellularization; Extracellular matrix; Macrophage polarization; Muscle flap fabrication; Vascularization; Volumetric muscle loss; Extracellular matrix; Muscle flap fabrication; Volumetric muscle loss; Macrophage polarization; Decellularization; Vascularization
• ISSN: 2452-199X; 2452-199X
• DOI: 10.1016/j.bioactmat.2022.10.023

Muscle flaps must have a strong vascular network to support a large tissue volume and ensure successful engraftment. We developed porcine stomach musculofascial flap matrix (PDSF) comprising extracellular matrix (ECM) and intact vasculature. PDSF had a dominant vascular pedicle, microcirculatory vessels, a nerve network, well-retained 3-dimensional (3D) nanofibrous ECM structures, and no allo- or xenoantigenicity. In-depth proteomic analysis demonstrated that PDSF was composed of core matrisome proteins (e.g., collagens, glycoproteins, proteoglycans, and ECM regulators) that, as shown by Gene Ontology term enrichment analysis, are functionally related to musculofascial biological processes. Moreover, PDSF−human adipose-derived stem cell (hASC) synergy not only induced monocytes towards IL-10−producing M2 macrophage polarization through the enhancement of hASCs' paracrine effect but also promoted the proliferation and interconnection of both human skeletal muscle myoblasts (HSMMs) and human umbilical vein endothelial cells (HUVECs) in static triculture conditions. Furthermore, PDSF was successfully prevascularized through a dynamic perfusion coculture of hASCs and HUVECs, which integrated with PDSF and induced the maturation of vascular networks in vitro. In a xenotransplantation model, PDSF demonstrated myoconductive and immunomodulatory properties associated with the predominance of M2 macrophages and regulatory T cells. In a volumetric muscle loss (VML) model, prevascularized PDSF augmented neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant musculofascial tissue formation. These results indicate that hASCs' integration with PDSF enhances the cells’ dual function in immunomodulation and angiogenesis. Owing in part to this PDSF-hASC synergy, our platform shows promise for vascularized muscle flap engineering for VML reconstruction. Prevascularized PDSF (engineered muscle flap) with a co-culture of human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs) was transplanted into a recipient nude rat for VML reconstruction. The inset image indicates the prevascularized area shown in the magnified view. [Display omitted] •This study showed a comprehensive proteomic analysis for a perfusable porcine muscle flap matrix (PDSF) that provides the molecular and topographical cues necessary for the integration and growth of multiple cell types.•PDSF-hASC synergy not only induces M2 macrophage polarization via the paracrine effects of hASCs but also promotes angiogenesis and vascular network formation and maturation.•A prevascularized muscle flap-stem cell construct, which includes a microcirculatory network and integrates multiple cell types, facilitates the constructive remodeling and long-term maintenance of muscle tissue regeneration for VML reconstruction.