Analysis of sequences from field samples reveals the presence of the recently described pepper vein yellows virus (genus Polerovirus) in six additional countries

• Published in: Archives of virology Vol. 158; no. 6; pp. 1337 - 1341
• Main Authors: Knierim, Dennis, Tsai, Wen-Shi, Kenyon, Lawrence
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.06.2013; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Brief Report; Capsicum; Capsicum - virology; Genome, Viral - genetics; Genomes; India; Indonesia; Infections; Infectious Diseases; Luteoviridae - genetics; Mali; Medical Microbiology; Philippines; Plant Diseases - statistics & numerical data; Plant Diseases - virology; Reverse Transcriptase Polymerase Chain Reaction; RNA polymerase; Sequence Analysis, DNA; Serology; Solanum nigrum; Solanum tuberosum; Taiwan; Thailand; Virology; Viruses; Thailand; Mali; Philippines; Taiwan; India; Indonesia; Japan; Yellow Virus; Potato Leaf Roll Virus; Reverse Transcription Polymerase Chain Reaction; Coat Protein Sequence; Pepper Plant
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-012-1598-y

Polerovirus infection was detected by reverse transcription polymerase chain reaction (RT-PCR) in 29 pepper plants ( Capsicum spp.) and one black nightshade plant ( Solanum nigrum ) sample collected from fields in India, Indonesia, Mali, Philippines, Thailand and Taiwan. At least two representative samples for each country were selected to generate a general polerovirus RT-PCR product of 1.4 kb length for sequencing. Sequence analysis of the partial genome sequences revealed the presence of pepper vein yellows virus (PeVYV) in all 13 samples. A 1990 Australian herbarium sample of pepper described by serological means as infected with capsicum yellows virus (CYV) was identified by sequence analysis of a partial CP sequence as probably infected with a potato leaf roll virus (PLRV) isolate.