Accumulation mechanisms and subcellular distribution of Cu in maize grown on soil treated with [S, S]-ethylenediamine disuccinic acid

• Published in: Plant and soil Vol. 351; no. 1-2; pp. 237 - 247
• Main Authors: Niu, Liyuan, Shen, Zhenguo, Luo, Chunling, Deng, Yue-e, Wang, Chunchun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.02.2012; Springer Netherlands; Springer Nature B.V
• Subjects: Acid soils; Acids; Agricultural research; Agricultural soils; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; apoplast; Biological and medical sciences; Biomedical and Life Sciences; Cell walls; Chelating agents; Copper; Corn; Ecology; Edetic acid; EDTA (chelating agent); Ethylenediamines; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Growth; Growth (Plants); Health aspects; Heavy metals; Leaves; Life Sciences; Membrane permeability; Membranes; Permeability; Physiological aspects; Phytoremediation; Plant growth; Plant Physiology; Plant roots; Plant Sciences; Plant-soil relationships; Plants; Regular Article; Roots; Shoots; Soil; Soil pollution; Soil science; Soil Science & Conservation; Soil treatment; Soil-plant relationships. Soil fertility; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Soils; Soils (acid); Xylem; Zea mays; China; Subcellular distribution; Maize; Membrane permeability; Cell compartment; Monocotyledones; Vegetals; Zea mays; C4-Type; Cereal crop; Accumulation; Mechanism; Soils; Acids; Gramineae; Angiospermae; Spermatophyta; Soil plant relation
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-011-0954-0

Aims Many studies have proved that EDTA (ethylenediaminetetraacetic acid), EDDS ([S, S']-ethylenediamine disuccinic acid), and other chelating agents significantly enhance phyto-extraction of copper (Cu) from soil. However, some key factors, such as changes in membrane permeability of root cells and subcellular distribution of Cu and Cu-EDDS complex in leaves and roots, remain unresolved. Methods A pot-culture experiment was conducted using soil artificially contaminated with Cu to different degrees to compare its effect on the above factors and the relationship between them in maize (Zea mays L.). Results Treatment with 0.5–6.0 mmol kg−1 (soil) EDDS increased membrane permeability in root cells significantly (p<0.05). Chelated Cu accounted for 14.6%–17.4% of the total Cu content of roots and 77.7%–78.8% of that of leaves and was distributed mainly in cell walls in both. Conclusions EDDS increases Cu accumulation in shoots mainly by increasing the content of soluble Cu in soil and membrane permeability of root cells. Cu in soil may be absorbed through the apoplastic pathway into the root xylem, translocated to the shoots, and accumulated there as a Cu-EDDS complex.