A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci

• Published in: Genomics (San Diego, Calif.) Vol. 85; no. 4; pp. 413 - 424
• Main Authors: Itoh, Tomohito, Watanabe, Toshio, Ihara, Naoya, Mariani, Paola, Beattie, Craig W., Sugimoto, Yoshikazu, Takasuga, Akiko
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.04.2005; Elsevier
• Subjects: Animals; bacterial artificial chromosomes; Biological and medical sciences; Cattle; Cattle - genetics; chromosome mapping; Classical genetics, quantitative genetics, hybrids; Comparative map; EST; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Genes. Genome; genetic markers; Genetics of eukaryotes. Biological and molecular evolution; Genome; Humans; loci; Methods, theories and miscellaneous; Microsatellite; microsatellite repeats; Microsatellite Repeats - genetics; Molecular and cellular biology; Molecular genetics; physical chromosome mapping; Radiation hybrid map; Radiation Hybrid Mapping; radiation hybrid maps; Microsatellite; Comparative map; Cattle; EST; Radiation hybrid map; Genetic mapping; Bovine; Genomics; Vertebrata; Mammalia; Microsatellite DNA; Artiodactyla; Genome; Locus; Ungulata
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2004.12.007

A bovine whole genome 7000-rad radiation hybrid (RH) panel, SUNbRH 7000-rad, was constructed to build a high-resolution RH map. The Shirakawa–USDA linkage map served as a scaffold to construct a framework map of 3216 microsatellites on which 2377 ESTs were ordered. The resulting RH map provided essentially complete coverage across the genome, with 1 cR 7000 corresponding to 114 kb, and a cattle–human comparative map of 1716 bovine genes and sequences annotated in the human genome, which covered 79 and 72% of the bovine and human genomes, respectively. We then integrated the bovine RH and comparative maps with BAC fingerprint information in http://www.bcgsc.ca/lab/mapping/bovine to construct a detailed, BAC-based physical map covering a reported 40-cM quantitative trait locus region for intramuscular fat or “marbling” on BTA 4. In summary, the new, high-resolution SUNbRH 7000-rad, comparative, Shirakawa–USDA linkage, and BAC fingerprint maps provide a set of genomic tools for fine mapping regions of interest in cattle.