Qualitative and Quantitative Analysis for the Chemical Constituents of Tetrastigma hemsleyanum Diels et Gilg Using Ultra-High Performance Liquid Chromatography/Hybrid Quadrupole-Orbitrap Mass Spectrometry and Preliminary Screening for Anti-Influenza Virus Components

• Published in: Evidence-based complementary and alternative medicine Vol. 2019; no. 2019; pp. 1 - 14
• Main Authors: Rong, Rong, Gong, LiLi, Yu, QinHui, Du, RuiKun, Liu, JiangTing, Ding, FuJuan, Jiang, Haiqiang
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2019; Hindawi; Hindawi Limited
• Subjects: Acids; Anthraquinones; Antitumor activity; Antiviral drugs; Biological activity; Chinese medicine; Chromatography; Constituents; Correlation analysis; Correlation coefficient; Correlation coefficients; Emodin; Esters; Fatty acids; Flavonoids; High performance liquid chromatography; Influenza; Ions; Kaempferol; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medical research; Medical screening; Medicinal plants; Phenols; Phytochemicals; Quadrupoles; Quantitative analysis; Quercetin; Rutin; Scientific imaging; Swine flu; Tetrastigma; Viruses; United States > US; China
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2019/9414926

Tetrastigma hemsleyanum Diels et Gilg (T. hemsleyanums) is a kind of traditional folk medicinal plant which has been used widely in China for its antivirus, antitumor, and other clinical effects. In this study, ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive/MS) was utilized to analyze the chemical constituents of T. hemsleyanums. Fifty-one constituents were clarified, including flavonoids, anthraquinones, esters, fatty acids, phenols, and catechins. In the subsequent quantitative analysis, the contents of ten compounds of rutin, kaempferol, astragalin, quercitrin, quercetin, vitexin-rhamnoside, isorhamnetin, vitexin, emodin-8-O-β-D-glucoside, and isoquercetin in 18 batches of T. hemsleyanums collected from different places of cultivation were determined. Meanwhile, anti-influenza virus bioactivity in vitro of the above samples was detected with Gaussia Luciferase viral titer assay. It was found that the antiviral bioactivity varied from batches to batches in accordance with content difference of the chemical constituents in T. hemsleyanums. Correlation analysis was performed with SPSS software for the association between LC-MS chemometrics and bioactivity of influenza virus inhibition, and 8 constituents of flavonoids showed positive correlation coefficient, which may provide a valuable clue for searching potential antiviral components in T. hemsleyanums.