Single conical track-etched nanopore for a free-label detection of OSCS contaminants in heparin

• Published in: Biosensors & bioelectronics Vol. 137; pp. 207 - 212
• Main Authors: Ma, Tianji, Balanzat, Emmanuel, Janot, Jean-Marc, Balme, Sébastien
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.07.2019; Elsevier
• Subjects: Chemical Sciences; Heparin; Nanopore; OSCS; Track-etched; Track-etched; OSCS; Nanopore; Heparin
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2019.05.005

The heparin contamination by oversulfated chondroitin (OSCS) was at the origin of one major sanitary problem of last decade. Here we propose a novel strategy to detect OSCS from heparin solution based on conical nanopore functionalized with poly-L-lysine deposition to ensure its re-usability. This sensor is an excellent to detect low heparin concentration (from 25 ng/ml to 3 μg/ml) using the modification of ionic current rectification. It also allows following the kinetic of heparin degradation by heparinase with a good correlation with results obtained by classical methods. The sensor is sensitive to the inhibition of heparinase by OSCS until a concentration of 200 pg/ml representing 0.01% in weight in a heparin. This resolution is one order of magnitude lower than the one obtained by chromatography. For the first time, it was reached without fluorescence labeling. [Display omitted] •We design conical nanopore with reversible functionalization based on poly-L-Lysine deposition.•We detect heparin from 25 ng/ml to 3 μg/ml using the ionic current rectification.•We follow the kinetic of heparin degradation by heparinase using nanopore detection.•We use this nanopore as sensor of OSCS until a concentration of 200 pg/ml representing 0.01% in weight in a heparin.