Genetic diversity of wild and cultured populations of Brycon opalinus (Cuvier, 1819) (Characiforme, Characidae, Bryconiae) using microsatellites

• Published in: Aquaculture Vol. 247; no. 1; pp. 51 - 65
• Main Authors: Melon Barroso, Renata, Hilsdorf, Alexandre W.S., Moreira, Heden L.M., Cabello, Pedro H., Traub-Cseko, Yara M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 30.06.2005; Elsevier Science; Elsevier Sequoia S.A
• Subjects: Agnatha. Pisces; Animal aquaculture; Animal productions; Biological and medical sciences; Brycon opalinus; Characidae; Characiformes; Fish; Freshwater; Fundamental and applied biological sciences. Psychology; Gene loci; General aspects; Genetic diversity; Microsatellite; Polymorphism; Population genetics; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Brazil, Paraiba; Microsatellite; Fish; Population genetics; Brycon opalinus; Polymorphism; Natural population; Genetic diversity; Biodiversity; Vertebrata; Pisces; Teleostei; Osteichthyes; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2005.02.004

The genetic structure of Brycon opalinus (Brazilian freshwater fish species) from seven wild populations across Paraíba do Sul basin and one cultured population was investigated using 7 microsatellite loci. Polymorphism at all loci was assessed with a total of 162 different alleles found. The observed heterozygosity ( H o) ranged from 0.23 to 1.00. Almost all populations, except from Itagaçaba River, deviated significantly to HWE showing deficit of heterozygote. All populations were characterized by high microsatellite variability (mean H=85.6%). Genetic structure was evidence by a significant F ST (0.0432). All pairwise distances ( F ST) were significant, suggesting that each population is a different unit of management. The hatchery, stocked since the 50s, showed the second great average allele number: 13.43 and a gene diversity ( H; observed with microsatellite markers) equal to 0.789. This was similar to previously published mitochondrial data ( h=0.753).