The Synthesis and Transfection Activity of Disulfide Polycationic Amphiphiles

Some new polycationic amphiphiles containing a disulfide group were synthesized. Cationic liposomes formed from the compounds synthesized and a helper lipid 1,2-dioleoyl- sn -glycero-3-phosphatidylethanolamine were not toxic for HEK293 and HeLa cells and were highly effective when delivering a fluor...

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Bibliographic Details
Published inRussian journal of bioorganic chemistry Vol. 49; no. 1; pp. 41 - 51
Main Authors Petukhov, I. A., Puchkov, P. A., Morozova, N. G., Zenkova, M. A., Maslov, M. A.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 2023
Springer Nature B.V
Subjects
Biochemistry
Biocompatibility
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
In vivo methods and tests
Life Sciences
Lipids
Liposomes
Organic Chemistry
Plasmids
Synthesis
disulfide bonds
polycationic amphiphiles
cationic liposomes
transfection
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Summary:Some new polycationic amphiphiles containing a disulfide group were synthesized. Cationic liposomes formed from the compounds synthesized and a helper lipid 1,2-dioleoyl- sn -glycero-3-phosphatidylethanolamine were not toxic for HEK293 and HeLa cells and were highly effective when delivering a fluorescently labeled oligodeoxyribonucleotide. The efficacy of plasmid DNA delivery depended on the cell line and the amphiphile structure, liposomes based on tetracationic amphiphiles being the most effective transfectants. These liposomes can be used for in vitro transfection of eukaryotic cells as well as for further in vivo biological studies.
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ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162023010235